Collagen Type I ELISA Kit (COLI)

Full Name: Collagen Type I ELISA Kit (COLI)
Reactivity: Human
Sample Type: Serum, Plasma, Tissue Homogenates, Biological Fluids
Sensitivity: 0.188 ng/ml

INTRODUCTION

Collagen type I (COLI) is regarded as one of the abundant molecules that is present within the body. They are vital in connective tissues, bones and skin. A key structural composition for many tissues, essentially an interstitial matrix that is organised into fibrils. to form collagen fibers. A single collagen molecule has three structural components: a protein domain, a triple helical domain and an amino acid-rich domain. The COLI domain is composed of amino acids arranged in three helices around the nonhelical region that contains most of the peptide bonds. The tropheil olefin is used to bond two opposite repeat units together, and his is known as the only hydrophobic bonding site within the molecule.

Collagen type I is synthesised as a pro-collagen precursor that is made up of a C-terminal pro-peptide, a central collagen domain, and an N-terminal pro-peptide. The pro-collagen chains can translocate to the lumen of the rough endoplasmic reticulum. This is followed a process of folding and post-translational modification within the ER, eventually the pro-collagen molecules are taken up by the Golgi apparatus and secreted out of the cell. The central domain of pro-collagen can be cleaved, resulting in either a procollagen or a metalloproteinase-resistant peptide. This is followed by ERAD, whereby the newly formed peptide is taken up into the lumen of the Golgi and transported. After transcytosis in a process called retrograde transport, pro-collagen reaches its destination: the extracellular matrix (ECM).

INTENDED USE

Human collagen type I ELISA kit is designed for analysing levels of collagen type I (COLI) present in human serum, biological fluids, tissue homogenates and plasma samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

One 96-Well Plate: Pre-coated with anti COLI antibody.
Standards: Lyophilized recombinant.
Sample/Standard Dilution Buffer.
Biotinylated-labelled Antibody.
Antibody Dilution Buffer.
HRP-Streptavidin Conjugate (SABC).
SABC Dilution Buffer.
TMB Substrate.
Wash Buffer (25x).
Plate Sealer.
Product Instructions.

TYPICAL RESULTS

For this collagen type I ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.312, 0.625, 1.25, 2.5, 5, 10, 20 ng/ml.
Reactivity: Human
Sensitivity: 0.188 ng/ml
Range: 0.313 – 20 ng/ml
Principle: Sandwich
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for COLI, no cross reactivity or interference between COLI and analogues was detected.
– Recovery: Serum (87 – 100%), EDTA Plasma (93 – 102%), Heparin Plasma (88 – 105%).
– Linearity: Serum (89 – 100%), EDTA Plasma (85 – 105%), Heparin Plasma (91 – 104%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

Evaluation of a Cell-Free Collagen Type I-Based Scaffold for Articular Cartilage Regeneration in an Orthotopic Rat Model. Materials (Basel). (2020) 13 (10): 2369. Szychlinska M.A., et al.
Structural basis of homo- and heterotrimerization of collagen I. Nat Commun. (2017) 8: 14671. Sharma U., et al.
Age-Related Alterations of Hyaluronan and Collagen in Extracellular Matrix of the Muscle Spindles. J Clin Med. (2021) 11 (1): 86. Fan C., et al.
Type I collagen expression contributes to angiogenesis and the development of deeply invasive cutaneous melanoma. Int J Cancer. (2008) 122 (5): 1019-29. van Kempen L.C., et al.
Effects of relaxin on collagen type I released by stretched human periodontal ligament cells. Orthod Craniofac Res. (2009) 12 (4): 282-8. Takano M., et al.

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