3-Methylhistidine ELISA Kit (3-MT)

INTENDED USE

Human 3-methylhistidine ELISA kit is designed for measuring concentrations of 3-methylhistidine (3MT, 3-MT) in human plasma, tissue homogenate, serum and other biological fluids. This assay has a minimum sensitivity detection limit of 3.7 nmol/ml.

INTRODUCTION

3-Methylhistidine (3-MT or 3MeH) is an amino acid that is found to be present in myosin and actin. This amino acid is regarded as a fundamental constituent of muscle proteins. It has been found in more than 90% of 3-MeH body pool that is present within the skeletal muscles.

During the process of skeletal muscle degradation, it is released and excreted through urine and it is not re-used for any other form of protein synthesis. This urinary secretion of 3-MT is considered as a useful indicator of muscle catabolism.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-3MT antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this 3-methylhistidine ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 6.25, 12.5, 25, 50, 100, 200, 400 nmol/ml.
Reactivity: Human
Sensitivity: 3.75 nmol/ml
Range: 6.25 – 400 nmol/ml
Principle: Competitive
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for 3MT, no cross reactivity or interference between 3MT and analogues was detected.
– Recovery: Serum (89 – 99%), EDTA Plasma (91 – 101%), Heparin Plasma (86 – 104%).
– Linearity: Serum (88 – 104%), EDTA Plasma (88 – 105%), Heparin Plasma (83 – 92%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. The effect of 3-methylhistidine in food on its urinary excretion in man. Clin Sci (Lond). (1980) 59 (6): 509-11. Elia M., et al.
2. Meat intake and risk of mortality and graft failure in kidney transplant recipients. Am J Clin Nutr. (2021) 114 (4): 1505-1517.
3. 3-Methylhistidine/creatinine ratio in urine from low-birth-weight infants. Clinical approach. Ann Nutr Metab. 1988;32(1):44-51. Hülsemann J., et al.

ADDITIONAL INFORMATION

• Full Name: 3-Methylhistidine ELISA Kit (3-MT)
• Reactivity: Human
• Sample Type: Plasma, Biological Fluids, Serum, Tissue Homogenates
• Sensitivity: 3.75 nmol/ml

OTHER RELATED ELISA KITS

• CAMP ELISA Kit
• Collagen Type I ELISA Kit
• Glycosaminoglycan ELISA Kit

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