3-Methylhistidine ELISA Kit (3-MT)

Full Name: 3-Methylhistidine ELISA Kit (3-MT)
Reactivity: Human
Sample Type: Plasma, Biological Fluids, Serum, Tissue Homogenates
Sensitivity: 3.75 nmol/ml


3-Methylhistidine (3-MT or 3MeH) is an amino acid that is found to be present in myosin and actin. This amino acid is regarded as a fundamental constituent of muscle proteins. It has been found in more than 90% of 3-MeH body pool that is present within the skeletal muscles.

During the process of skeletal muscle degradation, it is released and excreted through urine and it is not re-used for any other form of protein synthesis. This urinary secretion of 3-MT is considered as a useful indicator of muscle catabolism.


Human 3-methylhistidine ELISA kit is designed for measuring concentrations of 3-methylhistidine (3MT, 3-MT) in human plasma, tissue homogenate, serum and other biological fluids.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-3MT antibody.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.


For this 3-methylhistidine ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 6.25, 12.5, 25, 50, 100, 200, 400 nmol/ml.
Reactivity: Human
Sensitivity: 3.75 nmol/ml
Range: 6.25 – 400 nmol/ml
Principle: Competitive
Application: Research Use Only.


– Specificity: Highly specific for 3MT, no cross reactivity or interference between 3MT and analogues was detected.
– Recovery: Serum (89 – 99%), EDTA Plasma (91 – 101%), Heparin Plasma (86 – 104%).
– Linearity: Serum (88 – 104%), EDTA Plasma (88 – 105%), Heparin Plasma (83 – 92%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.


  1. The effect of 3-methylhistidine in food on its urinary excretion in man. Clin Sci (Lond). (1980) 59 (6): 509-11. Elia M., et al.
  2. Meat intake and risk of mortality and graft failure in kidney transplant recipients. Am J Clin Nutr. (2021) 114 (4): 1505-1517.
  3. 3-Methylhistidine/creatinine ratio in urine from low-birth-weight infants. Clinical approach. Ann Nutr Metab. 1988;32(1):44-51. Hülsemann J., et al.


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