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Chromogranin A (CgA) ELISA Kit

INTENDED USE

Human chromogranin A ELISA kit is intended for determining in vitro quantitative amounts of chromogranin A (CgA, parathyroid secretory protein-1) in human serum and plasma (EDTA). This assay has a minimum sensitivity limit of 5.0 µg/l.

BACKGROUND

Chromogranin A (CgA) which can also be known as parathyroid secretory protein-1, is part of the granins (chromogranin/secretogranin) family that are found located in secretory vesicles of neurons and endocrine cells (such as islet beta cell secretory granules in pancreas). There are many examples of cells that are able to produce CgA and these include beta cells of the pancreas, chromaffin cells of the adrenal medulla and enterochromaffin-like cells. Chromogranin A is also a precursor to a number of functional peptides for example catestatin, pancreastatin, parastatin and vasostatin, all of these peptides have a role in negatively modulating the neuroendocrine function of autocrine and paracrine cells.

Parathyroid secretory protein-1 is now also regarded as one of the most important circulating tumour marker many kinds of neuroendocrine tumours, its concentrations are found to be elevated in neuroblastoma, gastrinoma, gastroentero-pancreatic and pheochromocytoma tumors. Chromogranin A concentrations are found to display a high correlation to the tumour mass, this has led to it being used widely in order to monitor the outcome of a number of therapies. For example, an elevation in the levels of CgA in patients having prostate carcinoma hint at an unfavourable outcome of the disease. Parathyroid secretory protein-1 is elevated in pheochromocytomas and has also been discovered as an autoantigen in type 1 diabetes.

CHROMOGRANIN A ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Chromogranin A Microtiter Strips: Antibody precoated microwell plate in a resealable pouch with desiccant.
  • Standard (A-E): Concentrations 0, 30, 110, 450, 900 µg/l.
  • Control Low & High.
  • Antibody Conjugate: Rabbit anti-chromogranin A antibody, conjugated with peroxidase.
  • Substrate: Tetramethylbenzidine (TMB).
  • Wash Buffer Concentrate (50x).
  • Stop Solution: Causes severe skin burns and eye damage.

SENSITIVITY

The minimum detection sensitivity level of human chromogranin A (Parathyroid secretory protein-1 and CgA) using current chromogranin A ELISA kit was 5.0 µg/l. The dynamic range for this assay is 30.0 – 700.0 µg/l.

ASSAY CHARACTERISTICS

– Chromogranin A (CgA): ELISA
– Analytical Sensitivity: Limit of Detection (LOD)(Serum 6.5 µg/l), Limit of Quantification (LOQ) (Serum 7.4 µg/l).
– Intra-Assay Variations: 4.3 – 5.6%
– Inter-Assay Variations: 9.4 –11.0%
– Recovery: 93 – 96%
– Linearity: 94 – 109%
– High-Dose-Hook Effect: No hook effect was observed in samples with concentrations higher than 200,000 µg/l Chromogranin A.

REFERENCES

  1. A Critical Approach to Clinical Biochemistry of Chromogranin A. Adv Exp Med Biol. (2015) 867: 317-23. Review. Bottoni P., et al.
  2. Chromogranin A: a multifaceted cardiovascular role in health and disease. Curr Med Chem. (2012) 19 (24): 4042-50. Review. Angelone T., et al.
  3. Chromogranin A: any relevance in neuroendocrine tumors? Curr Opin Endocrinol Diabetes Obes. (2016) 23 (1): 28-37. Review. Kidd M., et al.
  4. Metabolic effects and mechanism of action of the chromogranin A-derived peptide pancreastatin. Regul Pept. (2010) 161 (1-3): 8-14. Review. Sánchez-Margalet V., et al.
  5. Catestatin: a multifunctional peptide from chromogranin A. Regul Pept. (2010) 162 (1-3): 33-43. Review. Mahata S.K., et al.

ADDITIONAL INFORMATION

  • Full Name: Chromogranin A (CgA) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma (EDTA)
  • Sensitivity: 5.0 µg/l

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