Feline Chlamydia ELISA Kit (Psittaci/Trachomatis)

Full Name: Feline Chlamydia ELISA Kit (Psittaci/Trachomatis)
Reactivity: Feline
Sample Type: Serum, Plasma
Sensitivity:  Qualitative

BACKGROUND

In majority of cases, chlamydia infections in feline can often result in either mouth or eye infections. This type of infection is treated similarly in both cats and humans. The signs are similar as well, including the presence of pus or discharge from the urethra, genitalia and eyes. In addition to that, there will also be swelling and a fever. To treat this type of infection cats need antibiotics for about 10 days to allow the organism time to clear out of their system.

Chlamydia are known to be able to form a long-term associations with the host cells. Vaccinated or chlamydia antigen infected cats have the ability to generate antibodies that are directed against these Chlamydia antigens. Cats that are infected with this Chlamydia but vaccinated against it can still generate antibodies. The species within “C. trachomatis” that is associated with the disease in cats is classified as “C. felis”.

INTENDED USE

Feline chlamydia ELISA kit is a method for analysing antibodies against chlamydia (psittaci/trachomatis)(cats) using serum or plasma samples.

 CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Coated Microtiter Strips.
• ELISA Buffer.
• Negative Control.
• Weak Positive Control.
• Strip Holder.
• HRPO Conjugated.
• Wash Solution (200x Concentrated).
• Substrate Buffer A.
• Substrate Buffer B.
• Plastic Cover Seal.
• Stop Solution.

SENSITIVITY

The standard range of antibodies against chlamydia (psittaci/trachomatis) using this feline chlamydia ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– OD of the weak positive control must be higher than 0.800.
– Sample is considered positive when the measured extinction is higher than 1.6 times the OD of the negative control.

REFERENCES

1. Evaluation of a monoclonal antibody based ELISA for detection of feline Chlamydia psittaci. Vet Rec. (1986) 119 (17): 418-20. Wills J.M., et al.
2. Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis. Infect Immun. (1981) 31 (3): 1161-76. Caldwell H.D., et al.
3. Monoclonal antibody to a major outer membrane protein of feline Chlamydia psittaci: antibody specificity and anti-idiotype antibody production. Vet Microbiol. (1994) 42 (1): 1-13. Ts’ao Y.C. and Magee W.E.
4. Plasmid diversity within the genus Chlamydia. J Gen Microbiol. (1989) 135 (5): 1145-51. Lusher M., et al.
5. Antigenic diversity of Chlamydia psittaci of mammalian origin determined by microimmunofluorescence. Infect Immun. (1985) 50 (3): 905-10. Perez-Martinez J.A. and Storz J.

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