Canine distemper virus IgG ELISA kit is a method for analysing IgG antibodies against canine distemper virus (CDV)(dog) in plasma or serum. This assay has a quantitative and qualitative standard range.
Canine distemper virus (CDV)(also referred to as hardpad disease) is a viral disease that can affect many different animal families such as dogs, wolves, foxes, coyotes, raccoons, ferrets, skunks and many large cats. In canines, distemper is found to impact several body systems, for example respiratory and gastrointestinal tracts, the brain and the spinal cord. Distemper virus can be caused by a single-stranded RNA virus that belong to the Paramyxoviridae family, it is also closely related to the viruses that result in rinderpest and measles. In order to prevent canine distemper, puppies need to be vaccinated at 6-8 weeks of age and then continue to have a “booster shot” every 2-4 weeks until reaching 16 weeks of age. The whole course of vaccination program needs to be undertaken, otherwise the vaccination will not provide any protection from the virus. Following assay is a monoclonal mediated procedure in order to measure IgG antibodies against canine distemper virus (CDV) in serum and plasma samples.
CANINE DISTEMPER VIRUS IGG ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microtiter Strips Coated With CDV Proteins.
- CDV Negative Control.
- CDV Positive Control.
- Strip Holder.
- HRPO-Conjugated (IgG)(Anti-Species) Antibody.
- ELISA Buffer.
- Wash Solution (200x Concentrated).
- Substrate Buffer A.
- Substrate Buffer B.
- Plastic Cover Seal.
- Stop Solution.
The standard range of IgG antibodies against CDV using current canine distemper virus (CDV) IgG ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.
– Canine Distemper Virus (CDV) IgG: ELISA
– Quantitatively (End Point Titer):
(i). < 30: No antibodies found.
(ii). 90 – 270: Antibodies found, probably shedding CCV, retest in 3 months.
(iii). > 810: High titer of antibodies found in recovered diseased animals. Diseased animal (suggestive for CDV). Healthy animal (retest in 3 months).
- Antigen requirements and specificity of enzyme-linked immunosorbent assay for detection of canine IgG against canine distemper viral antigens. Am J Vet Res. (1982) 43 (12): 2266-9. Bernard S.L., et al.
- Accuracy of a point-of-care ELISA test kit for predicting the presence of protective canine parvovirus and canine distemper virus antibody concentrations in dogs. Vet J. (2012) 193 (2): 363-6. Litster A.L., et al.
- Virological and serological findings in dogs with naturally occurring distemper. J Virol Methods. (2015) 213: 127-30. Elia G., et al.
- Detection of canine distemper virus antigen in canine serum and its application to diagnosis. Vet Rec. (2003) 153 (16): 499-501. Soma T., et al.
- Serological analysis of canine distemper virus using an immunocapture ELISA. J Vet Med Sci. (1996) 58 (8): 791-4. Gemma T., et al.
- Full Name: CDV (Canine Distemper Virus) IgG ELISA Kit (Dog)
- Reactivity: Dog
- Sample Type: Plasma, Serum
- Sensitivity: Quantitative, Qualitative