GET A QUOTE

CDV (Canine Distemper Virus) IgG ELISA Kit (Dog)

INTENDED USE

Canine distemper virus IgG ELISA kit is a method for analysing IgG antibodies against canine distemper virus (CDV)(dog) in plasma or serum. This assay has a quantitative and qualitative standard range.

BACKGROUND

Canine distemper virus (CDV)(also referred to as hardpad disease) is a viral disease that can affect many different animal families such as dogs, wolves, foxes, coyotes, raccoons, ferrets, skunks and many large cats. In canines, distemper is found to impact several body systems, for example respiratory and gastrointestinal tracts, the brain and the spinal cord. Distemper virus can be caused by a single-stranded RNA virus that belong to the Paramyxoviridae family, it is also closely related to the viruses that result in rinderpest and measles. In order to prevent canine distemper, puppies need to be vaccinated at 6-8 weeks of age and then continue to have a “booster shot” every 2-4 weeks until reaching 16 weeks of age. The whole course of vaccination program needs to be undertaken, otherwise the vaccination will not provide any protection from the virus. Following assay is a monoclonal mediated procedure in order to measure IgG antibodies against canine distemper virus (CDV) in serum and plasma samples.

CANINE DISTEMPER VIRUS IGG ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Strips Coated With CDV Proteins.
  • CDV Negative Control.
  • CDV Positive Control.
  • Strip Holder.
  • HRPO-Conjugated (IgG)(Anti-Species) Antibody.
  • ELISA Buffer.
  • Wash Solution (200x Concentrated).
  • Substrate Buffer A.
  • Substrate Buffer B.
  • Plastic Cover Seal.
  • Stop Solution.

SENSITIVITY

The standard range of IgG antibodies against CDV using current canine distemper virus (CDV) IgG ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Canine Distemper Virus (CDV) IgG: ELISA
– Quantitatively (End Point Titer):
(i). < 30: No antibodies found.
(ii). 90 – 270: Antibodies found, probably shedding CCV, retest in 3 months.
(iii). > 810: High titer of antibodies found in recovered diseased animals. Diseased animal (suggestive for CDV). Healthy animal (retest in 3 months).

REFERENCES

  1. Antigen requirements and specificity of enzyme-linked immunosorbent assay for detection of canine IgG against canine distemper viral antigens. Am J Vet Res. (1982) 43 (12): 2266-9. Bernard S.L., et al.
  2. Accuracy of a point-of-care ELISA test kit for predicting the presence of protective canine parvovirus and canine distemper virus antibody concentrations in dogs. Vet J. (2012) 193 (2): 363-6. Litster A.L., et al.
  3.  Virological and serological findings in dogs with naturally occurring distemper. J Virol Methods. (2015) 213: 127-30. Elia G., et al.
  4. Detection of canine distemper virus antigen in canine serum and its application to diagnosis. Vet Rec. (2003) 153 (16): 499-501. Soma T., et al.
  5. Serological analysis of canine distemper virus using an immunocapture ELISA. J Vet Med Sci. (1996) 58 (8): 791-4. Gemma T., et al.

ADDITIONAL INFORMATION

  • Full Name: CDV (Canine Distemper Virus) IgG ELISA Kit (Dog)
  • Reactivity: Dog
  • Sample Type: Plasma, Serum
  • Sensitivity: Quantitative, Qualitative

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford