Cardiotrophin-1 ELISA Kit (CT-1)

INTENDED USE

Human cardiotrophin-1 ELISA kit is intended for determining in vitro quantitative amounts of CT-1 (cardiotrophin-1) in human plasma, cell lysates, serum, other biological fluids and cell culture supernatant. This assay has a minimum sensitivity limit of 9.38 pg/ml.

BACKGROUND

Cardiotrophin-1 (CT-1) was initially discovered as a factor which was able to induce cardiac myocyte hypertrophy. It is a 21.5 kDa cardiac hypertrophic factor and a member of the interleukin-6 superfamily (also includes IL-11, LIF, CNTF and oncostatin M). It has been detected in abundance in adult human lung cells and expressed in both adult and fetal smooth airway muscle cells. It can cause increased levels of ventricular expression of ANP, BNP and angiotensinogen mRNA, this was significantly evident in patients with dilatative cardiomyopathy, heart failure, angina and mitral regurgitation.

Cardiotrophin-1 can exert its cellular effects by interacting with the gp130/LIF receptor beta (LIFR) heterodimer. It can activate PI-3 kinase in PI-3 kinase, whilst it also has the ability to enhance DNA-binding activities of the transcription factor NF-κB.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microwell Plate: CT-1 specific antibody pre-coated wells.
• Standards: Concentration ranging between 15.6 to 1,000 pg/ml.
• Sample/Standard Diluent Buffer.
• Wash Buffer.
• Detection Reagent A.
• Detection Reagent B.
• Diluent A.
• Diluent B.
• TMB Substrate.
• Stop Solution.
• Plate Sealer.

SENSITIVITY

The minimum detection sensitivity level of CT-1 (cardiotrophin-1) using this cardiotrophin-1 ELISA kit was 9.38 pg/ml. The dynamic assay range for this kit is 15.6 – 1,000.0 pg/ml.

ASSAY CHARACTERISTICS

– Intra Assay Precision: < 10%
– Inter Assay Precision: < 10%

REFERENCES

1. Cardiotrophin-1: A multifaceted cytokine. Cytokine Growth Factor Rev. (2015) 26 (5): 523-32. Review. López-Yoldi M., et al.
2. Vascular effects of glycoprotein130 ligands–part II: biomarkers and therapeutic targets. Vascul Pharmacol. (2012) 57 (1): 29-40. Review. Demyanets S., et al.
3. The Role of Leukemia Inhibitory Factor Receptor Signaling in Skeletal Muscle Growth, Injury and Disease. Adv Exp Med Biol. (2016) 900: 45-59. Review. Hunt L.C. and White J.
4. Cardiotrophin-1 (CT-1): a novel hypertrophic and cardioprotective agent. Int J Exp Pathol. (1999) 80 (4): 189-96. Review. Latchman D.S.

ADDITIONAL INFORMATION

• Full Name: Cardiotrophin-1 ELISA Kit (CT-1)
• Reactivity: Human
• Sample Type: Serum, Cell Lysates, Other Biological Fluids, Cell Culture Supernatant, Plasma
• Sensitivity: 9.38 pg/ml

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