BMP-5 ELISA Kit (Bone Morphogenetic Protein-5)
INTENDED USE
Human BMP-5 ELISA kit is a reliable quantitative procedure for measuring bone morphogenetic protein-5 (BMP5, BMP-5) in human serum, biological fluids, plasma and tissue homogenates. This assay has a minimum sensitivity detection limit of 0.094 ng/ml.
BACKGROUND
Bone morphogenetic protein 5 (BMP-5) is encoded by the BMP5 gene and can be mapped on the human chromosome 6.4. It is a member of the TGFβ superfamily, this superfamily is composed many growth and differentiation factors. The original identification of bone morphogenetic proteins was based on their ability to demineralized bone extract to induce endochondral osteogenesis (in vivo) in an extra skeletal site.
BMP-5 may play a certain role in specific cancers, it also like other bone morphogenetic proteins having ability to induce the development of bone and cartilage. Just like other BMP’s it can be inhibited by noggin and chordin, furthermore it is predominately expressed in the optic nerve head or in the trabecular meshwork, where it could have a role in normal function or in its development.
The standard used in the following assay is recombinant monomer that has a total molecular mass of 18KDa.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Microplate: Pre-coated with anti-BMP-5 antibody.
- Standard: Concentrations 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5, 10 ng/ml.
- Sample/Standard Diluent Buffer.
- HRP-Streptavidin Conjugate (SABC).
- Biotinylated Labelled Antobody (Concentrated).
- Antibody Diluent Buffer.
- SABC Dilution Buffer.
- TMB Substrate.
- Stop Solution.
- Wash Buffer (25x Conc.).
- Plate Sealer.
- Product Instructions.
SENSITIVITY
The minimum sensitivity detection limit of bone morphogenetic protein-5 (BMP-5, BMP5) using current BMP-5 ELISA kit was approximately 0.094 ng/ml. The dynamic range for this assay is 0.156 – 10.0 ng/ml.
ASSAY CHARACTERISTICS
– Specificity: Highly specific for detecting BMP-5, no cross reactivity or interference between other bone morphogenetic protein-5 analogues was detected.
– Recovery: Serum (92 – 103%), EDTA Plasma (85 – 102%), Heparin Plasma (87 – 103%).
– Linearity: Serum (85 – 105%), EDTA Plasma (82 – 99%), Heparin Plasma (80 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10% within the expiration date.
REFERENCES
- Epithelial-to-mesenchymal transition induced by TGF-β1 is mediated by Blimp-1-dependent repression of BMP-5. Cancer Res. (2012) 72 (23): 6268-78. Romagnoli M., et al.
- A bone morphogenetic protein subfamily: chromosomal localization of human genes for BMP5, BMP6, and BMP7. Genomics (1992) 14 (3): 759–62. Hahn G.V., et al.
- Bone morphogenetic proteins 5 and 6 stimulate osteoclast generation. J Biomed Mater Res A. (2006) 77 (1): 75-83. Wutzl A., et al.
ADDITIONAL INFORMATION
- Full Name: BMP-5 ELISA Kit (Bone Morphogenetic Protein-5)
- Reactivity: Human
- Sample Type: Serum, Plasma, Tissue Homogenates, Biological Fluids
- Sensitivity: 0.094 ng/ml
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