BMP-4 ELISA Kit (Bone Morphogenetic Protein-4)

INTENDED USE

Human BMP-4 ELISA kit is a reliable quantitative procedure for measuring bone morphogenetic protein-4 (BMP4, BMP-4) in human serum, plasma, tissue homogenates and biological fluids. This assay has a minimum sensitivity detection limit of 37.5 pg/ml.

BACKGROUND

Bone morphogenetic protein 4 (BMP-4, BMP4) is encoded by the BMP4 gene that is located on 14q22-q23.1. Some of its important functions include bone and cartilage development, especially limp and tooth development, in addition to repair of fractures. Other roles of included bone mineralization, muscle and uteric bud development, it has also been implicated in fibro dysplasia ossificans progressive where it is found to be under expressed. It can stimulate the differentiation of overlying ectodermal tissue whilst the inhibition of signal by chordin, follistatin or noggin is known to causes the ectoderm to differentiate into the neural plate.

BNP-4 is a critical signalling molecule in human embryonic development where it is required for early differentiation of the embryo and in establishing the dorsal-ventral axis. It is known to be secreted from the dorsal portion of the notochord and can act in concert with sonic hedgehog to establish a dorsal-ventral axis that can be used for the differentiation of later structures.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microplate: Pre-coated with anti-BMP-4 antibody.
• Standard: Concentrations 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml.
• Sample/Standard Diluent Buffer.
• Biotinylated Labelled Antobody (Concentrated).
• Antibody Diluent Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Stop Solution.
• Wash Buffer (25x Conc.).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum sensitivity detection limit of BMP-4 (bone morphogenetic protein-4, BMP4) using current BMP-4 ELISA kit was approximately 18.75 pg/ml. The dynamic range for this assay is 31.25 – 2000.0 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for detecting BMP-4, no cross reactivity or interference between other bone morphogenetic protein-4 analogues was detected.
– Recovery: Serum (86 – 105%), EDTA Plasma (88 – 100%), Heparin Plasma (91 – 103%).
– Linearity: Serum (86 – 103%), EDTA Plasma (82 – 101%), Heparin Plasma (81 – 97%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10% within the expiration date.

REFERENCES

1. Pituitary tumors: cell type-specific roles for BMP-4. Mol Cell Endocrinol. (2010) 326 (1-2): 85-8. Review. Labeur M., et al.
2. CCN3 increases BMP4 expression and bone mineralization in osteoblasts. J Cell Physiol. (2012) 227 (6): 2531-41. Tan T.W., et al.
3. A role for bone morphogenetic protein-4 in lymph node vascular remodeling and primary tumor growth. Cancer Res. (2011) 71 (20): 6547-57. Farnsworth R.H., et al.

ADDITIONAL INFORMATION

• Full Name: BMP-4 ELISA Kit (Bone Morphogenetic Protein-4)
• Reactivity: Human
• Sample Type: Tissue Homogenates, Plasma, Biological Fluids, Serum
• Sensitivity: 18.75 pg/ml

OTHER RELATED ELISA KITS

• BMP-2 ELISA Kit
• BMP-5 ELISA Kit
• VEGF ELISA Kit