Beta-2 Microglobulin ELISA Kit (B2MG)

INTENDED USE

Human beta-2 microglobulin ELISA kit can be used for determining in vitro quantitative amounts of human B2MG (beta-2-microglobulin, beta-2 microglobulin) in human serum, cell culture supernatant and various other biological samples. This assay has a minimum detection limit of < 0.2 μg/mL.

BACKGROUND

Beta-2 microglobulin (B2MG) is a 11 kDA protein that was originally identified as a small serum protein, this small size helps it to pass through the glomerular membrane and it is also often completely reabsorbed in the proximal tubules. It is able to form the subunit of the MHC class I molecule and also be associated with many outer membrane of a number of cells which includes lymphocytes.

Beta-2 microglobulin is usually present in the subunit of class I major histocompatibility complex (MHC I) which can be found expressed on all nucleated cell except red blood cells. It is also linked with MHC I-like molecules for example CD1 and Qa. Generally it is present in minute amounts but tends to increase its expression or even adapt to the fibrillary configuration depending on certain pathological states. Often B2MG is present at low levels in the serum or urine of normal people, however these concentrations are much higher in patients having kidney transplants, renal diseases and various other infectious or inflammatory conditions.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C in the dark. Do not expose reagents to heat, sun, or strong light during storage and usage.

• Microplate: Precoated with beta-2 microglobulin antigen.
• Calibrator A-F: Concentration 0-12 µg/ml.
• Control Positive and Control Negative (2).
• Sample Buffer PU (5x Concentrated).
• Enzyme Conjugate: Consisting of antibody conjugated to HRP.
• TMB Substrate.
• Stop Solution.
• Wash Buffer (50x Concentrated).
• Instructions
• Certificate of Analysis.

SENSITIVITY

The minimum detection sensitivity level beta-2 microglobulin (B2MG, beta-2-microglobulin) using current B2MG ELISA kit was 3.0 μg/mL. The dynamic range for this assay is 0.75 – 12.0 μg/mL.

REFERENCES

1. Beta2-microglobulin: emerging as a promising cancer therapeutic target. Drug Discov Today. (2009) 14 (1-2): 25-30. Review. Shi C., et al.
2. Solution structure of beta(2)-microglobulin and insights into fibrillogenesis. Biochim Biophys Acta. (2005) 1753 (1): 76-84. Review. Esposito G., et al.
3. Understanding the complex mechanisms of β2-microglobulin amyloid assembly. FEBS J. (2011) 278 (20): 3868-83. Review. Eichner T. and Radford S.E.
4. β2-Microglobulin-mediated signaling as a target for cancer therapy. Anticancer Agents Med Chem. (2014) 14 (3): 343-52. Review. Nomura T., et al.

ADDITIONAL INFORMATION

• Full Name: Beta-2 Microglobulin ELISA Kit (B2MG)
• Reactivity: Human
• Sample Type: Serum, Biological Samples, Cell Culture Supernatant
• Sensitivity: < 0.2 μg/mL

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