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Atrial Natriuretic Peptide (ANP) ELISA Kit

INTENDED USE

Human ANP ELISA kit is a procedure intended for determining in vitro quantitative amounts of atrial natriuretic peptide (ANP, atrial natriuretic factor, ANF, atrial natriuretic hormone, ANH) in human serum, cell lysates, plasma, other biological fluids and cell culture supernatant. This assay has a minimum sensitivity limit of 4.688 pg/ml.

BACKGROUND

Atrial natriuretic peptide (ANP) can also be referred to as atrial natriuretic hormone (ANH), atrial natriuretic factor (ANF), atriopeptin, cardionatrine and cardiodilatin. It is a 28 amino acid polypeptide hormone which is secreted by heart muscle cells, a powerful vasodilator. Human ANP is responsible for reducing the blood pressure by regulating homeostatic control of the body water, fat, sodium and potassium levels. It is often released by muscles cells in the atria of the heart in response to high blood volume. It can be secreted in response to raised sodium concentration, stretching of the atrial wall, endothelin, increased sympathetic stimulation of β-adrenoceptors and exercise.

Atrial natriuretic peptide is able to bind to a specific set of receptors which are called ANP receptors, this results in reducing blood volume, systemic blood pressure and a reduction in cardiac output. Generally, the function of ANP is to counter the increase in the volume and pressure of blood that is caused via the renin-angiotensin system.

The following atrial natriuretic peptide ELISA kit is an ideal tool for measuring concentrations of ANP, ANF and ANH using various different types of sample types. ANP and many of its related peptides are important biomarkers for many cardiovascular diseases such as coronary artery disease, stroke, heart failure and myocardial infarction. Also, recombinant atrial natriuretic peptide has been approved in Japan to be used in order to treat patients experiencing heart failure.

ANP ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human ANP antibody.
  • Standards: Concentrations 0, 7.812, 15.625, 31.25, 62.5, 125, 250, 500 pg/ml, made up from lyophilized recombinant human atrial natriuretic peptide.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled ANP Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

SENSITIVITY

The minimum detection sensitivity level of human atrial natriuretic peptide (ANH, atrial natriuretic hormone, ANP, ANF, atrial natriuretic factor) using current ANP ELISA kit was 4.688 pg/ml. The dynamic range for this assay is 7.813 – 500.0 pg/ml.

ASSAY CHARACTERISTICS

– Atrial Natriuretic Peptide (ANP): ELISA
– Specificity: Highly specific for ANP, no cross reactivity or interference between ANP and analogues was detected.
– Recovery: Serum (87 – 103%), EDTA Plasma (87 – 101%), Heparin Plasma (88 – 104%).
– Linearity: Serum (92 – 105%), EDTA Plasma (87 – 98%), Heparin Plasma (91 – 100%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. Human atrial natriuretic peptide treatment for acute heart failure: a systematic review of efficacy and mortality. Can J Cardiol. (2012) 28 (1): 102-9. Review. Kobayashi D., et al.
  2. Novel natriuretic peptides: new compounds and new approaches. Curr Heart Fail Rep. (2011) 8 (1): 22-7. Review. Vogel M.W and Chen H.H.
  3. Biomarkers and heart disease. Eur Rev Med Pharmacol Sci. (2014) 18 (19): 2927-35. Review. Sun R.R., et al.
  4. Roles of atrial natriuretic peptide and its therapeutic use. J Cardiol. (2010) 56 (3): 262-70. Review. Saito Y.
  5. Natriuretic peptides in the pathophysiology of congestive heart failure. Curr Cardiol Rep. (2000) 2 (3): 198-205. Review. Chen H.H. and Burnett J.C.

ADDITIONAL INFORMATION

  • Full Name: Atrial Natriuretic Peptide (ANP) ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Tissue Homogenates, Serum, Other Biological Fluids
  • Sensitivity: 4.688 pg/ml

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