Mycoplasma Pneumoniae IgG ELISA Kit

Full Name: Mycoplasma Pneumoniae IgG ELISA Kit
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: 1.12 U/ml


Mycoplasma pneumoniae are a class of mollicutes which are responsible for causing the human disease mycoplasma pneumonia. There are a form of atypical bacterial pneumonia which are known to be related to the cold agglutinin disease. They are parasitic species that are void of periplasmic space and a cell wall, consist of a reduce genome, have limited metabolic activity and are the smallest self-replicating organisms.

Mycoplasma pneumoniae cells are made up of an elongated shape. It can also cause tracheobronchitis, pharyngitis, otitis or other or community-acquired pneumonia, however, it may remain totally asymptomatic.


Human Mycoplasma pneumoniae IgG ELISA kit is a procedure for measuring in-vitro levels of human IgG antibodies against Mycoplasma pneumoniae (Mycoplasma-IgG) in plasma and serum. This assay has a minimum analytical sensitivity limit of 1.12 U/ml.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Mycoplasma pneumoniae antigen coated microtiter strips.
  • Positive Control (Calibrator D).
  • Negative Control (Calibrator A).
  • Cut-Off Standard (Calibrator B).
  • Weak Positive Control (Calibrator C).
  • Enzyme Conjugate.
  • Sample Diluent.
  • TMB Substrate.
  • Washing Buffer (10x).
  • Stop Solution.


The minimum detection sensitivity level of human IgG antibodies to Mycoplasma pneumoniae using current Mycoplasma IgG ELISA kit was 1.12 U/ml. The dynamic range for this assay is 1.0 – 100.0 U/ml.


  1. IgGresponse to Mycoplasma pneumoniaein patients with community-acquired pneumonia determined by ELISA. APMIS. (1988) 96 (7): 605-10. Hirschberg L., et al.
  2. The atypical pneumonias: clinical diagnosis and importance. Clin Microbiol Infect. (2006) 12 Suppl 3: 12-24. Review. Cunha B.A.
  3. Evaluation of four commercial IgG- and IgM-specific enzyme immunoassays for detecting Mycoplasma pneumoniae-antibody: comparison with particle agglutination assay. J Korean Med Sci. (2007) 22 (5): 795-801. Yoo S.J., et al.
  4. IgG-mediated haemolysis masquerading as cold agglutinin-induced anaemia complicating severe infection with mycoplasma pneumoniae. Scand J Haematol. (1985) 35 (3): 264-9. Louie E.K., et al.
  5. Preparative electroelution of specific protein antigens from Mycoplasma pneumoniae: use in an enzyme-linked immunosorbent assay (ELISA). Ann Inst Pasteur Microbiol. (1988) 139 (5): 589-603. Papierok G., et al.


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