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Alpha MSH ELISA Kit (Alpha Melatonin Stimulating Hormone)

Full Name: Alpha MSH ELISA Kit (Alpha Melatonin Stimulating Hormone)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 7.5 pg/ml

INTRODUCTION

Alpha Melanocyte stimulating hormone (alpha MSH) has a tridecapeptide structure and the amino acid sequence of Ac-Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-NH 2. As a hormonal peptide, the physiological function consists mainly in the control of pigment movements within dermal melanophores. There is evidence it functions as a mediator of immunity and inflammation. The peptide is produced, stored and secreted in alpha cells of the suprarenal medulla termed as chromaffin cells. It signals to other parts of the body with melanocytes, which are cells that produce pigment, to increase melanin production. As a result, it can have an effect on the skin’s colour and appearance. The physiological function consists mainly in controlling pigment movements within dermal melanophores and immune responses. The levels are correlated many diseases and health conditions including; hypertension, carcinoma, rheumatoids arthritis, obesity, type-2 diabetes, coronary heart disease and Alzheimer’s disease. The substance was first identified in rats as an endogenous pyrogen, which is said to induce fever and inflammation.

During puberty, the body’s levels of alpha MSH increase, causing the gradual darkening of skin colour. Melanin is a pigment that helps protect the body from UV radiation by absorbing it and dissipating it more safely. It also prevents too much absorption of UV rays, which would lead to sunburns. In addition to being a pigment, melanin also acts as an antioxidant and can neutralise free radicals that are present in the skin. Free radicals can cause cellular damage and age the skin prematurely.

INTENDED USE

Human alpha MSH ELISA kit can be used to determine levels of alpha melatonin stimulating hormone (alpha MSH, AMSH) present is human serum, tissue homogenates, plasma and other biological fluids.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-alpha-MSH antibody.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

TYPICAL RESULTS

For this alpha MSH ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 12.5, 25, 50, 100, 200, 400, 800 pg/ml.
Reactivity: Human
Sensitivity: 7.5 pg/ml
Range: 12.5 – 800 pg/ml
Principle: Competitive
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for AMSH, no cross reactivity or interference between AMSH and analogues was detected.
– Recovery: Serum (87 – 99%), EDTA Plasma (88 – 105%), Heparin Plasma (87 – 105%).
– Linearity: Serum (86 – 102%), EDTA Plasma (82 – 91%), Heparin Plasma (80 – 94%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

  1. The neuroimmunomodulatory peptide alpha-MSH. Ann N Y Acad Sci. (2000) 917: 221-6. Ichiyama T., et al.
  2. Alpha-melanocyte-stimulating hormone and behavior. Neurosci Biobehav Rev. (1982) 6 (3): 297-310. Datta P.C., and King M.G.
  3. Mechanisms of Melatonin in Alleviating Alzheimer’s Disease. Curr Neuropharmacol. (2017) 15 (7): 1010-1031. Shukla M., et al.
  4. Mechanisms of antiinflammatory action of alpha-MSH peptides. In vivo and in vitro evidence. Ann N Y Acad Sci. (1999) 885: 173-82. Lipton J.M., et al.

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