Alpha Amylase Saliva ELISA Kit

INTENDED USE

Human alpha amylase saliva ELISA kit is an accurate in vitro quantitative method for detecting alpha-amylase (alpha amylase, α-amylase) in human saliva samples. This assay has a minimum sensitivity detection limit of 3.6 U/ml.

BACKGROUND

Alpha amylase (α-amylase) is a digestive enzyme which is responsible for breaking down starch to maltose. This is carried out by hydrolysing alpha-1,4 bonds of large polysaccharides such as starch and glycogen, also yielding smaller by-products such as glucose and maltose. It is predominately synthesised in the acinar cells of the saliva glands and can be stored in secretory granules inside the cells. Since it has the ability to act anywhere on the substrate, it is found to be faster acting when compared to than β-amylase. In animals, it functions as the major digestive enzyme which requires an optimum pH is 6.7–7.0.

In addition to its digestive function, alpha amylase can also play vital anti-bacterial roles in the oral cavities and for this reason alone it can be stored at much lower levels in non-stimulated saliva between meals. However, the release by salivary cells can be greatly increased in response to the chewing motions of the jaw or taste. The production by saliva glands can be elevated in response to physical and psychological stress via interactions with the autonomic nervous system.

CONTENT

All reagents provided need to be stored at a temperature between +2°C to +8°C. Do not freeze. Unopened, all test kit components are stable until the indicated expiry date.

• Microplate: Wells coated with alpha-amylase antigen.
• Calibrator 1-6:  Concentration 0 – 500 U/ml.
• Control 1 and Control 2.
• Antiserum: Polyclonal antibody (rabbit).
• Enzyme Conjugate.
• Sample Buffer.
• Wash Buffer (10x).
• TMB Chromogen Solution.
• Stop Solution.
• Protective Foil.
• Test Instruction.
• Quality Control Certificate.

SENSITIVITY

The minimum sensitivity detection limit of alpha amylase (alpha-amylase, α-amylase) using current salivary alpha amylase ELISA kit was approximately 3.6 U/ml. The dynamic range for this assay is 10.0 – 50.0 U/ml.

ASSAY CHARACTERISTICS

– Detection Limit: 3.6 U/ml
– Cross Reactivity: Alpha amylase in human saliva (100%), Porcine pancreatic alpha amylase (< 0.23%), Alpha amylase from Bacillus sp. (< 0.01%).
– Interference: None
– Intra-Assay Variation: 3.6 – 5.5%
– Inter-Assay Variation: 4.2 – 9.6%
– Linearity: Linear detection between the concentration range 12 to 500 U/ml.
– Correlation: A comparison against several reference tests yielded a correlation value range of 0-958 U/ml.

REFERENCES

1. Cortisol and α-Amylase Secretion Patterns between and within Depressed and Non-Depressed Individuals. PLoS One. (2015) 10 (7): e0131002. Booij S.H., et al.
2. Salivary amylase – The enzyme of unspecialized euryphagous animals. Arch Oral Biol. (2015) 60 (8): 1162-76. Review. Boehlke C., et al.
3. Increased salivary immunoglobulin A and reduced α-amylase activity in whole saliva from spastic cerebral palsy individuals. J Oral Pathol Med. (2013) 42 (6): 480-5. Leite M.F., et al.
4. Salivary alpha-amylase in biobehavioral research: recent developments and applications. Ann N Y Acad Sci. (2007) 1098: 122-44. Review. Granger D.A., et al.
5. Determinants of salivary alpha-amylase in humans and methodological considerations. Psychoneuroendocrinology. (2009) 34 (4): 469-85. Review. Rohleder N. and Nater U.M.

ADDITIONAL INFORMATION

• Full Name: Alpha Amylase Saliva ELISA Kit
• Reactivity: Human
• Sample Type: Saliva
• Sensitivity: 3.6 U/ml

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