Free Cortisol Saliva ELISA Kit


Free cortisol saliva ELISA kit is an accurate in vitro quantitative method for detecting free cortisol in any species using saliva samples. This assay has a minimum sensitivity detection limit of 24.0 pg/ml.


Cortisol that can also be called compound F hydrocortisone is produced in the adrenal cortex within the zona fasciculate or the zona reticularis. Majority of it is found to bound either to cortisol-binding globulins (CBG)(around 90%) or albumin (approx. 7%), whilst only a small fraction between 1-3% is found either as unconjugated or free. This is the biologically active form, it is released in salvia and is then excreted through the kidneys as a relatively small portion among many of the metabolites.

In humans, the level of cortisol physiologically fluctuate throughout the day, where the highest concentration are found in the morning and the lowest at midnight. This different levels of fluctuations are accurately reflected in saliva where the highest peak is observed within the first 90 minutes after waking up. Due to the fact that collecting saliva samples is relatively easy and is non-invasive, without putting the patient under any unnecessary stress due to any repeated venipunctures. This assay offers an excellent alternative to LC-MS/MS method, it is simple to perform and provides accurate results that are comparable.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiterwells (96 Wells): Coated with polyclonal rabbit anti-cortisol antibody.
  • Calibrator 0.
  • Calibrators 1-5: Concentrations 0.1, 0.4, 1.7, 7.0, 30 ng/ml.
  • Control 1 (Low) And Control 2 (High).
  • Enzyme Conjugate: Consisting of antibody conjugated to horseradish peroxidase (HRP).
  • TMB Substrate Solution.
  • Stop Solution
  • Wash Solution (10x Concentrated).


The minimum sensitivity detection limit of free cortisol using current cortisol saliva ELISA kit was approximately 24.0 pg/ml. The dynamic range for this assay is 0.1 – 30.0 ng/ml.


– Analytical Sensitivity: 0.019 ng/ml
– Specificity (Cross Reactivity): Prednisolone (100%), 11-Deoxycortisol (50%), Corticosterone (6.2%), 11-Deoxycorticosterone (2.6%), 17-Hydroxyprogesterone (1.3%), Prednisone (0.9%), Cortisone (0.8%), Testosterone, Dexamethasone, Estriol, Estrone, Progesterone, Danazole, Pregnenolone, Estradiol, Androstenedione (< 0.1%).
– Assay Dynamic Range: 0.1 – 30 ng/ml.
– Intra-Assay Variation: 4.1 – 7.1%
– Inter-Assay Variation: 4.2 – 9.1%
– Recovery: 92 – 126%
– Linearity: 99 – 120%


  1. Measurement of salivary-cortisol in 2012 – laboratory techniques and clinical indications. Clin Endocrinol (Oxf). (2012) 77 (5): 645-51. Review. Inder W.J., et al.
  2. Saliva cortisol–a new approach in noise research to study stress effects. Int J Hyg Environ Health. (2005) 208 (3): 227-30. Review. Bigert C., et al.
  3. Salivary cortisol for assessment of hypothalamic-pituitary-adrenal axis function. Neuroimmunomodulation. (2009) 16 (5): 284-9. Review. Törnhage C.J.
  4. Salivary-cortisol in psychobiological research: an overview. Neuropsychobiology. (1989) 22 (3): 150-69. Review. Kirschbaum C. and Hellhammer D.H.


  • Full Name: Free Cortisol Saliva ELISA Kit
  • Reactivity: Any Species
  • Sample Type: Saliva
  • Sensitivity: 24.0 pg/ml


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