GET A QUOTE

Adiponectin ELISA Kit

INTENDED USE

Human adiponectin ELISA kit is a reliable quantitative procedure for measuring adiponectin (AdipoQ, Acrp30, GBP-28) in human serum or heparin plasma samples. This assay has a sensitivity detection limit of 0.6 ng/ml.

BACKGROUND

Adiponectin which can also be called Acrp30, GBP-28, AdipoQ is a recently identified 30kDa protein that is 244 amino acids in length. It is a product of the apM1 gene that is present in 0.01% of total serum proteins and is mainly synthesised by adipocytes, however, there is evidence that it can also be produced by hepatocytes and muscle cells. Adiponectin belongs to the superfamily of soluble defence collagen proteins where it contains a collagen-like domain that is domain structurally homologous with complement factor C1q-like globular domain, collagen VIII and collagen X. Until recently, IGF-I was the only known natural inductor for its synthesis which possess a globular C-terminal domain and a collagen-like N-terminal. Also, up to now there have been two different receptors that have been cloned adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2), where AdipoR1 is found to be expressed abundantly in skeletal muscle, whereas AdipoR2 is expressed predominantly in the liver.

The concentration of human adiponectin is found to correlates negatively with glucose, insulin, liver fat content, body mass index and triglyceride concentrations. Furthermore, it correlates positively with hepatic insulin-stimulated glucose disposal, insulin sensitivity and high-density lipoprotein cholesterol levels. Beside the correlation with BMI, adiponectin level are associated with insulin-resistance, linked with type II diabetes, involved in inflammatory processes, associated also with glucose and lipometabolism. On the whole, human adiponectin has the potential to become a clinically relevant parameter which can be used to routinely measure individuals that are at risk for type 2 diabetes, metabolic syndrome and atherosclerosis.

ADIPONECTIN ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Coated with mouse anti-adiponectin antibody. Wells are separately breakable.
  • Standards A-E: Concentrations 0, 2, 10, 30, 70, 100 ng/mL, made up from lyophilized native human adiponectin.
  • Control Serum 1 And Control Serum 2 (Lyophilised).
  • Antibody-HRP-Conjugate: Consisting of mouse anti-hAdiponectin antibody biotinylated and streptavidin horseradish peroxidase conjugate.
  • Dilution Buffer.
  • Washing Buffer (20x Concentrated).
  • Substrate: Horseradish-peroxidase- (HRP) substrate, stabilised Tetramethylbencidine.
  • Stopping Solution.
  • Sealing Tape: To cover microtiter plate.
  • Instructions.
  • Quality Certificate.

SENSITIVITY

The minimum sensitivity detection limit of human adiponectin (Acrp30, GBP-28, AdipoQ) using current adiponectin ELISA kit was approximately 0.6 ng/ml. The dynamic range for this assay is 2.0 – 100.0 ng/ml.

ASSAY CHARACTERISTICS

– Adiponectin (Acrp30, AdipoQ): ELISA
– Intra-Assay Variance: 3.14 – 3.97%
– Inter-Assay Variance: 6.93 – 8.16%
– Linearity: No deviation of the mean > 30% was detected.
– Recovery: 101 – 116%
– Interference: None

REFERENCES

  1. Adiponectin and atherosclerotic disease. Clin Chim Acta (2004) 344 (1-2): 1-12. Shimada K., et al.
  2. Induction of adiponectin in skeletal muscle by inflammatory cytokines: in vivo and in vitro studies. Endocrinology (2004) 145 (12): 5589-97. Delaigle A.M., et al.
  3. Adiponectin and leptin gene polymorphisms in patients with post-transplant diabetes mellitus. Pharmacogenomics. (2015) 16 (11): 1243-51. Romanowski M., et al.
  4. Adiponectin and cardiovascular inflammatory responses. Curr Atheroscler Rep. (2007) 9 (3): 238-43. Takemura Y., Walsh K. and Ouchi N.
  5. Adiponectin–a key adipokine in the metabolic syndrome. Diabetes Obes Metab. (2006) 8 (3): 264-80. Whitehead J.P., et al.

ADDITIONAL INFORMATION

  • Full Name: Adiponectin ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma (Heparin)
  • Sensitivity: 0.6 ng/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford