Adiponectin ELISA Kit (AdipoQ, Acrp30)

Quick Summary

Intended Use: Quantitative measurement of human adiponectin (AdipoQ, Acrp30, GBP-28) in serum and heparin plasma.

Sensitivity: 0.6 ng/ml | Range: 2.0 – 100.0 ng/ml

Sample Types: Human serum, Heparin plasma

INTENDED USE

Human adiponectin ELISA kit is a reliable quantitative procedure for measuring adiponectin (AdipoQ, Acrp30, GBP-28) in human serum or heparin plasma samples. This assay has a sensitivity detection limit of 0.6 ng/ml.

BACKGROUND

Adiponectin (APN, AdipoQ, Acrp30) is predominately expressed within adipose tissues, one of the main functions involves increasing insulin resistance. This is demonstrated by a decrease in the levels present in plasma for individuals with coronary artery disease, obesity and type 2 diabetes. This is not just found in humans but also in rodents.

The structure involves a complex of protein dimers and tetramers. There are two specific types of receptors AdipoR1 and AdipoR2. AdipoR1 is mainly expressed within skeletal muscles, it is responsible for enhancing fatty acid oxidation and promoting insulin sensitivity via activation of AMP-activated protein. Whereas, AdipoR2 is predominately expressed within the liver, it is responsible for enhancing metabolism of energy and oxidation of fatty acids via activation of PPARγ.

Adiponectin is composed of 244 amino acids, it contains a collagen domain (N-terminal) and globular domain (C-terminal). The N-terminal is found to be conserved among various species and plays an important role in the formation of multimers. It circulates in the blood as multiple isoforms, these play vital roles by influencing the process of inflammation during the chronic and acute phase diseases.

KIT CONTENTS & STORAGE

Storage Note: All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Component	Description
Microtiter Plate	Coated with mouse anti-adiponectin antibody. Wells are separately breakable.
Standards A-E	Concentrations 0, 2, 10, 30, 70, 100 ng/mL.
Control Serum 1 & 2	Lyophilised.
Antibody-HRP-Conjugate	Mouse antibody biotinylated and streptavidin horseradish peroxidase conjugate.
Dilution Buffer	For sample and reagent dilution.
Washing Buffer	20x Concentrated.
Substrate	Horseradish-peroxidase- (HRP) substrate, stabilised Tetramethylbenzidine.
Stopping Solution	For reaction termination.
Sealing Tape	To cover microtiter plate during incubation.
Instructions & Quality Certificate	Complete protocol and quality assurance documentation.

ASSAY PERFORMANCE CHARACTERISTICS

Parameter	Specification
Sensitivity (Detection Limit)	0.6 ng/ml
Dynamic Range	2.0 – 100.0 ng/ml
Intra-Assay Variance	3.14 – 3.97%
Inter-Assay Variance	6.93 – 8.16%
Linearity	No deviation of the mean > 30% was detected.
Recovery	101 – 116%
Interference	None

SPECIFICATIONS SUMMARY

Parameter	Detail
Full Name	Adiponectin ELISA Kit (AdipoQ, Acrp30)
Reactivity	Human
Sample Type	Serum, Plasma (Heparin)
Sensitivity	0.6 ng/ml
Assay Range	2.0 – 100.0 ng/ml

REFERENCES

1. Induction of adiponectin in skeletal muscle by inflammatory cytokines: in vivo and in vitro studies. Endocrinology (2004) 145 (12): 5589-97. Delaigle A.M., et al.

2. Adiponectin_and_leptin gene polymorphisms in patients with post-transplant diabetes mellitus. Pharmacogenomics. (2015) 16 (11): 1243-51. Romanowski M., et al.

3. Adiponectin and cardiovascular inflammatory responses. Curr Atheroscler Rep. (2007) 9 (3): 238-43. Takemura Y., Walsh K. and Ouchi N.

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