VEGF ELISA Kit (Vascular Endothelial Growth Factor)

INTENDED USE

Human VEGF ELISA kit is a reliable quantitative procedure for measuring vascular endothelial growth factor (VEGF) in human serum, tissue homogenates, biological fluids and plasma. This assay has a minimum sensitivity detection limit of 18.75 pg/ml.

BACKGROUND

Vascular endothelial growth factor (VEGF) is a member of the platelet-derived growth factor family of cystine-knot growth factors. It binds to tyrosine kinase receptors present on the cell surface. This leads to the formation of dimers and activation by transphosphorylation. The VEGFR consists of an extracellular portion that is made up of 7 immunoglobulin-like domains, a single transmembrane spanning region and an intracellular portion which consists of a split tyrosine-kinase domain.

In addition to binding to VEGFRs, there is evidence that VEDF can also bind to receptor complexes consisting of both VEGFRs and neuropilins. This receptor complex is found to result in an increase in the signalling activity in blood vessels (endothelial cells). There are essentially three main subtypes of VEGFR which can be numbered 1, 2 and 3. There is also a membrane-bound (mbVEGFR) or soluble (sVEGFR). It is a vital signalling protein that is found to be involved in both angiogenesis and vasculogenesis.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti VEGF antibody.
• Standards: Concentrations 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml.
• Sample/Standard Dilution Buffer.
• Biotinylated-Labelled-Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

SENSITIVITY

The minimum sensitivity detection limit of vascular endothelial growth factor (VEGF) using this VEGF ELISA kit was approximately 18.75 pg/ml. The dynamic assay range 31.25 – 2000.0 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for VEGF, no cross reactivity or interference between VEGF and analogues was detected.
– Recovery: Serum (92 – 105%), EDTA Plasma (92 – 102%), Heparin Plasma (85 – 101%).
– Linearity: Serum (89 – 104%), EDTA Plasma (82 – 101%), Heparin Plasma (83 – 98%).
– Precison Intra-Assay: CV < 8%.
– Precison Inter-Assay: CV < 10%.
– Stability: Less than 10%.

REFERENCES

1. Inhibiting angiogenesis with human single-chain variable fragment antibody targeting VEGF. Microvasc Res. (2015) 97: 13-8. Hosseini H., et al.
2. Vascular endothelial growth factor a inhibition in gastric cancer. Gastric Cancer. (2015) 18 (1): 33-42. Review. Park D.J., et al.
3. Distinct VEGF functions during bone development and homeostasis. Arch Immunol Ther Exp (Warsz). (2014) 62 (5): 363-8. Review. Liu Y. and Olsen B.R.

ADDITIONAL INFORMATION

• Full Name: VEGF ELISA Kit (Vascular Endothelial Growth Factor)
• Reactivity: Human
• Sample Type: Serum, Biological Fluids, Plasma, Tissue Homogenates
• Sensitivity: 18.75 pg/ml

OTHER RELATED ELISA KITS

• TGF Alpha ELISA Kit
• Activin A ELISA Kit
• PLGF ELISA Kit