Neutrophil ELISA Kit (NE)

INTENDED USE

Human neutrophil ELISA kit is ideal for detecting NE (neutrophil) in human samples of plasma or serum. This assay has a minimum sensitivity detection limit of 0.156 ng/ml.

INTRODUCTION

Neutrophil (NE) are important during inflammation and early combat against pathogens such as viruses, bacteria and fungi. They play a key role in mediating the anti-microbial activities via an elaborate process of releasing toxic factors, enzymes, generating re-active oxygen species and releasing neutrophil extracellular traps (NETs). Other functions include the ability to sense blood pH changes via their osmotic proton pumps (OPTs). They can help with wound-healing processes such as in burn patients or those who have undergone organ transplantation.

Neutrophil are representing between 50-75% of total circulating leukocytes that are present within the blood. Selectin and integrins play a key function in neutrophils exiting the blood and then entering in other tissues, this process is called diapedesis. Those unable to migrate to other tissues will undergo apoptosis and eventually be discarded by the body using macrophages. The lifespan is dependent on the following factors: Number of leukocytes in circulation (increased by active migration), presence or lack of oxygen and availability of nutrients.

Neutrophil have a short lifespan; it will undergo apoptosis when limited by oxygen and nutrients. The functions include fighting off infection, destroying bacteria, defending against pathogens and possibly serving as a messenger between cells to secrete signalling molecules that can trigger an immune response or attract other inflammatory cells. White blood cells help fight infection, destroy bacteria, defend against pathogens and possibly serve as messengers.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtitre Strips (96 Wells): Coated with mouse monoclonal antibody against human neutrophil.
• 10x Wash Buffer.
• 5x Assay Buffer.
• 100x Detection Antibody Buffer: Labelled biotin polyclonal antibody.
• Standard: Recombinant lyophilized.
• 200x STP-HRP Solution.
• Substrate Solution.
• Stop Solution.

TYPICAL RESULTS

For the following neutrophil ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5, 10 ng/ml.

ASSAY CHARACTERISTICS

– Sensitivity: 0.156 ng/ml.
– Assay Range: 0.156 – 10 ng/ml.
– Specificity: No interference or cross reaction observed.
– Other Names: Leukocyte elastase, Neutrophil, Elaszym, Serine elastase, ELANE.

REFERENCES

1. The double-edged role of neutrophil_extracellular traps in inflammation. Biochem Soc Trans. (2019) 47 (6): 1921-1930. Euler M. and Hoffmann M.H.
2. Leukocyte elastase: physiological functions and role in acute lung injury. Am J Respir Crit Care Med. (2001) 164 (5): 896-904. Lee W.L. and Downey G.P.
3. The enigmatic neutrophil: what we do not know. Cell Tissue Res. (2018) 371 (3): 399-406. Kubes P.
4. The elastase-induced expression of secretory leukocyte protease inhibitor is decreased in remodelled airway epithelium. Eur J Pharmacol. (1997) 336 (2-3): 187-96. Marchand V., et al.
5. Mechanisms of Inflammation in Neutrophil Mediated Skin Diseases. Front Immunol. (2019) 10: 1059. Marzano A.V., et al.
6. Repurposing a leukocyte elastase inhibitor for neuropathic pain. Nat Med. (2015) 21 (5): 429-30. Weyer A.D. and Stucky C.L.

ADDITIONAL INFORMATION

• Full Name: Neutrophil ELISA Kit (NE)
• Reactivity: Human
• Sample Type: Plasma, Serum
• Sensitivity: 0.156 ng/ml

OTHER RELATED ELISA KITS

• Galactin-3 ELISA Kit
• Haptoglobin ELISA Kit
• GDF-15 ELISA Kit

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