Mouse N-ERC/Mesothelin ELISA Kit

Full Name: Mouse N-ERC/Mesothelin ELISA Kit
Reactivity: Mouse
Sample Type: Plasma, Cell Culture Supernatant, Serum
Sensitivity: 0.15 ng/ml

BACKGROUND

ERC was first discovered as a gene that was expressed highly in cancer affected renal tissue when compared to normal renal tissues in Eker rats. The megakaryocyte potentiating factor (MPF) or the mesothelin are the equivalent human homologue of this protein. The CERC or the mesothelin gene is a tumor suppressor gene that is translocated to the DNA of cancer cells. When this gene has been put in error, it “promotes cell survival and progression. ”This protein controls how many megakaryocytes are produced in bone marrow. It also helps regulate hematopoiesis and allows for a balanced amount of red blood cells and platelets. Mesothelin or CERC is found on chromosome 8 at position 19q13.2-19q13.1. M-mesothelin and S-mesothelin are the two different forms of mesothelin genes. The m-mesothelin has four functions: it promotes cell survival, it regulates megakaryocyte production, it helps regulate hematopoiesis and red blood cells, and it allows for a balanced amount of platelets. Mutations in the CERC gene lead to overexpression of this protein that causes an increase in blood cell counts in newly formed RBCs.As a result, the chance of malignant growths on the surface of organs like the kidney or lung is enhanced. When there is more mesothelin in the bloodstream, it can be transported in the body and then cause inflammation or cancerous growths.

Mesothelin testing is indicated for a suspected mesothelioma case. Mesothelin is a protein with the molecular weight of approximately 24kDa. It can be detected in urine, serum or cerebrospinal fluid and is predominantly present in plasma. The m-mesothelin has four functions: it promotes cell survival, it regulates megakaryocyte production, it helps regulate hematopoiesis and red blood cells, and it allows for a balanced amount of platelets. Mutations in the CERC gene lead to overexpression of this protein that causes an increase in blood cell counts in newly formed RBCs. This leads to an increase in megakaryocyte production, which can lead to a blood cell anemia.

INTENDED USE

Mouse N-ERC/Mesothelin ELISA kit is a protocol intended for analysing quantitative levels of N-ERC or mesothelin in mouse samples of cell supernatant, serum and plasma. This assay has a minimum sensitivity detection limit of 0.15 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Pre-Coated Plate: Anti-mouse ERC (150) rabbit IgG.
• Labelled Antibody HRP Conjugated (30x Conc.).
• Standard.
• EIA Buffer.
• Solution For Labelled Antibody.
• TMB Chromogen.
• Stop Solution.
• Wash Buffer (Concentrated).

SENSITIVITY

The minimum sensitivity detection limit of N-ERC (mesothelin) and mesothelin using this mouse N-ERC/Mesothelin ELISA kit is 0.15 ng/ml. The dynamic range for this assay is 0.5 – 32.0 ng/ml.

ASSAY CHARACTERISTICS

– Mouse N-ERC (Mesothelin): ELISA
– Added Recovery Assay: 74.9 – 119.4%
– Intra – Assay: 4.1 – 9.4%
– Inter – Assay: 6.0 – 10.8%
– Specificity: Mouse N-ERC/Mesothelin (100.0%), Mouse C-ERC/Mesothelin (<0.1%).
– Sensitivity: 0.15 ng/mL

REFERENCES

1. Novel ELISA system for detection of N-ERC/mesothelin in the sera of mesothelioma patients. Cancer Sci. (2006) 97 (9): 928-32. Shiomi K., et al.
2. The utility of serum N-ERC /mesothelin as a biomarker of ovarian carcinoma. Oncol Lett. (2012) 4 (4): 637-641. Saeki H., et al.
3. Sensitive and specific new enzyme-linked immunosorbent assay for N-ERC/ mesothelin increases its potential as a useful serum tumor marker for mesothelioma. Clin Cancer Res. (2008) 14 (5): 1431-7. Shiomi K., et al.

OTHER RELATED ELISA KITS

• N-ERC/Mesothelin ELISA Kit (Rat)
• Osteopontin ELISA Kit (Rat)
• Osteopontin N-Half ELISA Kit (Mouse)