CDV IgM ELISA Kit (Canine Distemper Virus)

Full Name: CDV IgM ELISA Kit (Canine Distemper Virus)
Reactivity: Canine/Dog
Sample Type: Serum, Plasma
Sensitivity: Qualitative

BACKGROUND

Canine distemper virus (CDV), can also be sometimes called hardpad disease. It is caused by single stranded DNA virus, a member of the Paramyxoviridae family and related to the viruses that can lead to measles or rinderpest. This can affect various animal families for example; dogs, foxes, wolves, ferrets, coyotes, skunks and raccoons. Especially in canines, CDV can impact many different body systems such as the brain, spinal cord, gastrointestinal tracts and respiratory tracts. A distemper virus that affects these various body systems can cause a significant impact on an individual. If the individual is a human, it can cause significant neurological damage. Even if it does not affect the brain, distemper virus can impact other systems of the body including causing breathing difficulties or gastrointestinal problems such as vomiting and diarrhea.

Vaccination from CDV is important in order to prevent this disease, puppies need to be vaccinated initially between 6-8 weeks, followed by a booster shot every 2-4 weeks until age of 16 weeks. The following protocol is based on a monoclonal mediated system that can determine IgM antibodies against canine distemper virus (CDV) using samples of plasma and serum.

INTENDED USE

CDV IgM ELISA kit is designed for measuring IgM antibodies to canine distemper virus (CDV)(dog) using plasma or serum.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter Strips Coated With Monoclonal Anti-Distemper Virus Antibody.
Negative Control.
Positive Control.
Strip Holder.
Anti-Dog IgM HRPO Conjugated.
ELISA Buffer.
Wash Solution (200x Concentrated).
Substrate Buffer A.
Substrate Buffer B.
Plastic Cover Seal.
Stop Solution.

SENSITIVITY

The standard range of IgM antibodies to canine distemper virus (CDV) using current CDV IgM ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Test is valid if the first two dilutions of the positive control are above 0.200 (OD 450 nm).
– Negative control should give an OD < 0.200.

REFERENCES

Evaluation of a dot ELISA kit for measuring immunoglobulin M antibodies to canine parvovirus and distemper virus. Vet Rec. (2003) 152 (19): 588-91. Waner T., et al.
Detection of IgM antibodies against canine distemper virus in dog and mink sera employing enzyme-linked immunosorbent assay (ELISA). J Vet Diagn Invest. (1991) 3 (1): 3-9. Blixenkrone-Møller M., et al.
Immunohistochemical analysis of the lymphoid organs of dogs naturally infected with canine distemper virus. J Comp Pathol. (1995) 113 (2): 185-90. Iwatsuki K., et al.
The use of monoclonal antibodies specific for seal immunoglobulins in an enzyme-linked immunosorbent assay to detect canine distemper virus-specific immunoglobulin in seal plasma samples. J Immunol Methods. (1993) 160 (2): 163-71. King D.P., et al.

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