EBV EBNA-1 IgM ELISA Kit (Epstein–Barr Nuclear Antigen 1)

INTENDED USE

Human EBV EBNA-1 IgM ELISA kit can be used for analysing in-vitro levels of human IgM antibodies against Epstein–Barr virus EBNA-1 (EBV EBNA-1 IgM) in plasma and serum samples. This assay has a minimum analytical sensitivity limit of 1.09 U/ml.

BACKGROUND

Epstein–Barr nuclear antigen 1 (EBNA1) was the first Epstein-Barr virus (EBV) protein which was detected and is probably one of the most widely studied, it is expressed in both lytic and latent modes of EBV-infections. It displays a number of properties which include formation of dimers, sequence specific binding to DNA, multifunctional phosphorylation, the stimulation of cellular gene expression and nuclear localisation.

The expression of EBV-encoded proteins has been found to be most stringently restricted in primary Burkitt’s lymphoma where only EBNA1 and the non-translated Epstein-Barr virus-encoded small nuclear RNAs are transcribed. It consists of a glycine–alanine repeat sequence and this separates the protein into the amino- and carboxy-terminal domains, other roles for this sequence include preventing proteasomal breakdown, stabilising the protein, antigen processing and MHC class I-restricted antigen presentation. Many studies have indicated a vital role in maintaining tumor function.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• EBV EBNA-1 coated microtiter strips.
• Enzyme Conjugate.
• Sample Diluent.
• TMB Substrate.
• Washing Buffer (10x).
• Stop Solution.
• Calibrator B (Cut-Off Standard).
• Calibrator D (Positive Control).
• Calibrator C (Weak Positive Control).
• Calibrator A (Negative Control).

SENSITIVITY

The minimum detection sensitivity level of human anti-IgM against Epstein–Barr virus EBNA-1 (EBV-EBNA-1 IgM) using current EBV EBNA-1 IgM ELISA kit was 1.09 U/ml. The dynamic range for this assay is 1.0 – 150.0 U/ml.

ASSAY CHARACTERISTICS

– Intra-Assay-Precision: 7.8 %
– Inter-Assay-Precision: 12.1 %
– Inter-Lot-Precision: 4.5 – 12.9 %
– Analytical Sensitivity: 1.09 U/mL
– Recovery: 82 – 118 %
– Linearity: 76 – 119 %
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL.
– Cross-Reactivity: No cross-reactivity to Cytomegaly, Herpes and Varicella.
– Clinical Specificity: 98 %
– Clinical Sensitivity: 100 %

REFERENCES

1. The rapid diagnosis of infectious mononucleosis using an ELISA that detects IgM antibody to a peptide component of Epstein-Barr virus nuclear antigen. Diagn Microbiol Infect Dis. (1991) 14 (4): 287-91. Levin M.J., et al.
2. Mapping an epitope in EBNA-1 that is recognized by monoclonal antibodies to EBNA-1 that cross-react with dsDNA. Immun Inflamm Dis. (2016) 4 (3): 362-75. Yadav P., et al.
3. [Evaluation of IgG-IgM antibodies against the Epstein-Barr virus nuclear antigen (EBNA-1) using ELISA]. Enferm Infecc Microbiol Clin. (1992) 10 (5): 281-3. Spanish. Martínez Zapico R., et al.
4. Identical IgM antibodies recognizing a glycine-alanine epitope are induced during acute infection with Epstein-Barr virus and cytomegalovirus. J Clin Lab Anal. (1990) 4 (6): 456-64. Rhodes G., et al.

ADDITIONAL INFORMATION

• Full Name: EBV EBNA-1 IgM ELISA Kit (Epstein–Barr Nuclear Antigen 1)
• Reactivity: Human
• Sample Type: Serum, Plasma
• Sensitivity: 1.09 U/ml

OTHER RELATED ELISA KITS

• EBV EBNA-1 IgG ELISA Kit
• EBV VCA IgM ELISA Kit
• EBV EA IgM ELISA Kit

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