Glucagon ELISA Kit

INTENDED USE

Human glucagon ELISA kit can detect amounts of glucagon (glicentin, GC) present is human plasma, serum, biological fluids and tissue homogenates. This assay has a sensitivity detection limit of 4.688 pg/ml.

INTRODUCTION

Glucagon is an important peptide hormone which is produced by the alpha cells that are found in the pancreatic islets, its main function is that of a counter-regulatory partner to the hormone insulin. While insulin functions in the lowering of blood sugar after eating a meal, glucagon is the antagonist and it is responsible for preventing the blood glucose levels from dropping too low, this is known as condition called hypoglycemia. However, when a body is in that state fasting state or when it is under intense physical stress, this causes the pancreas release glucagon into the bloodstream. The released glucagon mainly travels to the liver, where it is responsible for triggering glycogenolysis. This is the breakdown of stored glycogen into glucose and gluconeogenesis which is the synthesis of glucose from other non-carbohydrate sources such as amino acids. By the process of stimulating this pathway, glucagon is important is ensuring that the primary organs such as the brain and the muscles have a continuous supply of fuel (glucose), thereby maintaining the systemic homeostasis during periods when energy is in a deficit state.

Furthermore, to its important role in glucose mobilisation, glucagon has a significant influence on lipid metabolism and energy expenditure. Through the process of signalling the liver to shift away from fatty acid synthesis and to move towards fatty acid oxidation, glucagon can promote the production of ketone bodies. This is vital and it serves as another source of energy during period of prolonged fasting or when carbohydrate is restricted. This kind of metabolic flexibility is important for survival especially during periods of nutrient scarcity. Recently, glucagon has be known to have a role as a thermogenic and this can reduce appetite, many leading researchers are investigating its potential in therapies for weight management. In addition, when it is used in combination with the GLP-1 receptor agonists, glucagon-based treatments have been used with the aim to balance blood sugar control by increasing the caloric burn, this offers a more sophisticated approach in the treatment of obesity and various metabolic dysfunction.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-glucagon antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Stop Solution
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this glucagon ELISA kit it is recommended that a standard curve is generated for each assay carried out.

-> Standard Curve: 0, 7.812, 15.625, 31.25, 62.5, 125, 250, 500 pg/ml.
-> Reactivity: Human
-> Sensitivity: 4.688 pg/ml
-> Range: 7.813 – 500 pg/ml
-> Principle: Sandwich
-> Application: Research Use Only.

ASSAY CHARACTERISTICS

–> Specificity: Highly specific for GC, no cross reactivity or interference between GC and analogues was detected.
–> Recovery: Serum (85 – 101%), EDTA Plasma (86 – 105%), Heparin Plasma (87 – 98%).
–> Linearity: Serum (88 – 105%), EDTA Plasma (86 – 102%), Heparin Plasma (85 – 104%).
–> Precison Intra-Assay: CV < 5%.
–> Precison Inter-Assay: CV < 5%.

REFERENCES

1. Hepatic glucagon action: beyond glucose mobilization. Physiol Rev. (2024) 104 (3):1021-1060. Kajani S, et al.
2. Glucagon, from past to present: a century of intensive research and controversies. Lancet Diabetes Endocrinol. (2023) 11(2):129-138. Scheen A.J and Lefèbvre P.J.
3. Glucagon-receptor antagonism requires further mechanistic attention on alpha-cell physiology.Trends Endocrinol Metab. (2025) 36 (9):785-788. Jia J., et al.
4. Glucagon in glucose homeostasis and metabolic disease: from physiology to therapeutics. J Basic Clin Physiol Pharmacol. (2025) 36 (2-3):139-146. Amin A. and Salman T.M.

ADDITIONAL INFORMATION

• Full Name: Glucogan ELISA Kit
• Reactivity: Human
• Sample Type: Serum, Tissue Homogenates, Plasma, Biological Fluids
• Sensitivity: 4.688 pg/ml

OTHER RELATED ELISA KITS

• Insulin ELISA Kit
• C-Peptide ELISA Kit
• Leptin ELISA Kit

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