8-OHdG ELISA Kit (8-Hydroxy-2-Deoxyguanosine)

INTENDED USE

Human 8-OHdG ELISA kit is an accurate in vitro quantitative method for detecting 8-hydroxy-2-deoxyguanosine (8-OHdG, 8-OHdG check) in human saliva, serum, urine and plasma samples. This assay has a minimum sensitivity detection limit of 0.5 ng/ml.

BACKGROUND

8-hydroxy-2-deoxyguanosine (8-OHdG) is a modified base in the DNA as a result of an attack by hydroxyl radicals. These are formed has either intermediates or products during the process of oxidative stress or aerobic metabolism. There are many publications which support the involvement of free radical reactions in the DNA damage process of many biomolecules which can ultimately lead to many diseases in humans for example cancer, diabetes, neurodegenerative conditions (Alzheimer’s disease), rheumatoid arthritis, atherosclerosis, inflammation, cerebral and heart ischemia-reperfusion injury.

The measurement of 8-OHdG levels is becoming increasingly popular as a stable, sensitive and an integral marker for oxidative damage in cellular DNA. It can be excreted through urine and that its levels can be elevated if exposed to ionising radiation and tobacco smoke. It has been found that the concentration correlates well with the damage to DNA and oxidative stress that leads to the degenerative disease states.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtiter Plate: Pre-coated with anti-8­-OHdG antibody (8×12wells, split type).
• Primary Antibody: Contains monoclonal antibody (clone N45.1).
• Primary Antibody Solution.
• Secondary Antibody: HRP­ conjugated anti mouse antibody.
• Secondary Antibody Solution.
• TMB Chromatic Solution.
• Diluting Solution.
• Washing Solution (5x).
• Reaction Terminating Solution.
• Standards: Concentration 0 – 200 ng/mL.
• Plate Seal.

SENSITIVITY

The minimum sensitivity detection limit of human 8-hydroxy-2-deoxyguanosine (8-OHdG) using current 8-OHdG ELISA kit was approximately 0.5 ng/ml. The dynamic assay range for this kit is 0.5 – 200.0 ng/ml.

REFERENCES

1. Determination of 8-hydroxydeoxyguanosine in human cells under oxygen-free conditions. Carcinogenesis. (1996) 17 (4): 787-91. Nakajima M., et al.
2. Oxidative damage to mitochondrial DNA and its relationship to ageing. Int J Biochem Cell Biol. (1995) 27 (7): 647-53. Review. Richter C.
3. Why and how should we measure oxidative DNA damage in nutritional studies? How far have we come? Am J Clin Nutr. (2000) 72 (5): 1082-7. Review. Halliwell B.
4. Can oxidative DNA damage be used as a biomarker of cancer risk in humans? Problems, resolutions and preliminary results from nutritional supplementation studies. Free Radic Res. (1998) 29 (6): 469-86. Review. Halliwell B.

ADDITIONAL INFORMATION

• Full Name: 8-OHdG ELISA Kit (8-Hydroxy-2-Deoxyguanosine)
• Reactivity: Human
• Sample Type: Saliva, Plasma, Urine, Tissue, Other Biological Samples
• Sensitivity: 0.5 ng/ml

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