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Serum Amyloid A ELISA Kit

INTENDED USE

Human serum amyloid ELISA kit can be used for detecting in vitro quantitative concentrations of serum amyloid A (SAA) in human plasma, serum, cell culture supernatant and many other biological fluids. This assay has a minimum detection limit of < 1.1 ng/ml.

BACKGROUND

Serum amyloid A (SAA) is a 104 amino acid polypeptide which in its native state has a molecular mass between 12-14 kDa. It is also a precursor of amyloid A (AA) protein (approx. 8.5 kDa) that can be produced following the cleavage of the first 76 amino acids of SAA. The gene is found to be located on chromosome 11p and the human protein can exist in a number of polymorphic forms (SAA1 to SAA4). The serum amyloid A has been identified as an acute-phase protein, which during acute events results in rapid elevation of SAA levels, in addition to an intense increase in all the other acute phase proteins. Many cytokines for example IL-1, IL-6 and TNF are considered to be mediators of SAA protein synthesis, they have the ability to stimulate hepatocytes in the liver in order to produce and release SAA within the bloodstream. This human SAA ELISA kit can be be used for analysing various different types of samples for example; many biological fluids, serum, plasma, cell supernatant.

In general SAA circulates at trace levels (between levels of 1-5 µg/mL) under normal conditions; however following 4-6 hours after an inflammatory stimulus, this causes the SAA levels to rapidly elevate to greater than 1000 fold (approx. 500-1000 µg/mL). This response of serum amyloid A makes it a very sensitive marker for many inflammatory stimulus. High levels of SAA are found in patients that experience acute and chronic inflammation. Also, there is evidence that secondary amyloidosis may develop if there is a prolonged or repeated inflammatory conditions where the levels of SAA remain elevated. Inflammatory disorders such as rheumatoid arthritis, ankylosing spondylitis, progressive sclerosis (tuberculosis or osteomyelitis), juvenile arthritis and familial mediterranean fever are pre-disposed to developing amyloidosis. Therefore determining the serum amyloid A levels in these patients can provide a useful indicator of degree of inflammation and their response to any therapy. The following SAA ELISA kit is designed for many different diagnostic applications.

SERUM AMYLOID A ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiterwells: Break apart strips, coated with anti human serum amyloid A antigen.
  • Standards 0-5: Lyophilized human SAA. Concentration 0-80 ng/ml.
  • Controls 1 and Control 2: Contains human serum amyloid A.
  • Enzyme Conjugate: Consisting of anti human SAA antibody conjugated to horseradish peroxidase (HRP).
  • Substrate Solution: Tetramethylbenzidine (TMB).
  • Stop Solution: Contains 1 N acidic solution, Avoid contact with the stop solution. It may cause skin irritations and burns.
  • Wash Solution (Concentrated).

SENSITIVITY

The minimum detection sensitivity level of serum amyloid A using current human SAA ELISA kit was 1.1 ng/ml. The dynamic range for this assay is 1.1 – 80 ng/ml.

REFERENCES

  1. Serum amyloid A (SAA): a concise review of biology, assay methods and clinical usefulness. Clin Chem Lab Med. (1999) 37 (4): 381-8. Review. Yamada T.
  2. Emerging functions of serum amyloid A in inflammation. J Leukoc Biol. (2015) 98 (6): 923-9. Review. Ye R.D. and Sun L.
  3. SAA, an apoprotein of HDL: its structure and function. Ann N Y Acad Sci. (1982) 389: 183-9. Review. Benditt E.P., et al.
  4. The cytokine-serum amyloid A-chemokine network. Cytokine Growth Factor Rev. (2016) 30: 55-69. Review. De Buck M., et al.
  5. Immune functions of serum amyloid A. Crit Rev Immunol. (2012) 32 (4): 335-48. Review. Eklund K.K., et al.

ADDITIONAL INFORMATION

  • Full Name: Serum Amyloid A ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma, Cell Culture Supernatant, Other Biological Fluid
  • Sensitivity: < 1.1 ng/ml

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