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Serum Amyloid A (SAA) ELISA Kit

  • Created on the 22 March, 2017.

BACKGROUND

Serum amyloid A (SAA) is a 104 amino acid polypeptide which in its native state has a molecular mass between 12-14 kDa. It is also a precursor of amyloid A (AA) protein (approx. 8.5 kDa) that can be produced following the cleavage of the first 76 amino acids of SAA. The gene is found to be located on chromosome 11p and the human protein can exist in a number of polymorphic forms (SAA1 to SAA4). The serum amyloid A has been identified as an acute-phase protein, which during acute events results in rapid elevation of SAA levels, in addition to an intense increase in all the other acute phase proteins. Many cytokines for example IL-1, IL-6 and TNF are considered to be mediators of SAA protein synthesis, they have the ability to stimulate hepatocytes in the liver in order to produce and release SAA within the bloodstream.

In general SAA circulates at trace levels (between levels of 1-5 µg/mL) under normal conditions; however following 4-6 hours after an inflammatory stimulus, this causes the SAA levels to rapidly elevate to greater than 1000 fold (approx. 500-1000 µg/mL). This response of SAA makes it a very sensitive marker for many inflammatory stimulus. High levels of SAA are found in patients that experience acute and chronic inflammation. Also, there is evidence that secondary amyloidosis may develop if there is a prolonged or repeated inflammatory conditions where the levels of SAA remain elevated. Inflammatory disorders such as rheumatoid arthritis, ankylosing spondylitis, progressive sclerosis (tuberculosis or osteomyelitis), juvenile arthritis and familial mediterranean fever are pre-disposed to developing amyloidosis. Therefore determining the SAA levels in these patients can provide a useful indicator of degree of inflammation and their response to any therapy.

INTENDED USE

Human SAA ELISA kit can be used for detecting in vitro quantitative concentrations of serum amyloid A (SAA) in human plasma, serum, cell culture supernatant and many other biological fluids. This assay has a minimum detection limit of < 1.1 ng/ml.

SENSITIVITY

The minimum detection sensitivity level of serum amyloid A using this human SAA ELISA kit was 1.1 ng/ml. The dynamic assay range for this kit is 1.1 – 80 ng/ml.

REFERENCES

  1. Serum amyloid A (SAA): a concise review of biology, assay methods and clinical usefulness. Clin Chem Lab Med. (1999) 37 (4): 381-8. Review. Yamada T.
  2. Emerging functions of serum amyloid A in inflammation. J Leukoc Biol. (2015) 98 (6): 923-9. Review. Ye R.D. and Sun L.
  3. SAA, an apoprotein of HDL: its structure and function. Ann N Y Acad Sci. (1982) 389: 183-9. Review. Benditt E.P., et al.
  4. The cytokine-serum amyloid A-chemokine network. Cytokine Growth Factor Rev. (2016) 30: 55-69. Review. De Buck M., et al.
  5. Immune functions of serum amyloid A. Crit Rev Immunol. (2012) 32 (4): 335-48. Review. Eklund K.K., et al.

ADDITIONAL INFORMATION

  • Full Name: Serum Amyloid A (SAA) ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma, Cell Culture Supernatant, Other Biological Fluid
  • Sensitivity: < 1.1 ng/ml

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