- Created on the 7 February, 2017.
Human insulin ELISA kit is a reliable quantitative procedure for measuring insulin in human serum and plasma. This assay has a minimum sensitivity detection limit of 0.25 µIU/ml.
Insulin is a polypeptide hormone that is secreted from the pancreas by beta cells of the islets of Langerhans, it has a molecular weight of 5800 Da and plays an important role in regulating carbohydrate metabolism. Insulin is also known to possess a wide spectrum of biological actions and these include: increasing DNA replication and protein synthesis, controlling the cellular intake of glucose in adipose tissue and muscles, fatty acid synthesis, allosteric effect on numerous enzyme, gluconeogenesis, decreased proteinolysis, amino acid uptake, lipolysis and increased glycogen. Insulin secretion can be mainly controlled by the levels of plasma glucose where hyperglycemia is known to induce a prompt increase in levels of insulin in circulation. Other factors which can also have an influencing factor include neural, metabolic and hormonal (glucagon, amino acids and gastrointestinal hormone) can all participate in controlling the levels of insulin that is being secreted.
However, there are some conditions where insulin disturbance is pathologic such as metabolic syndrome, diabetes mellitus, polycystic ovary syndrome and insulinoma. Diabetes mellitus exists in two forms: type 1 where in the conditions of absolute insulin deficiency there is an autoimmune-mediated destruction of insulin producing beta cells in the pancreas and type 2 where this form is strongly inherited which is the result of a combined influence of genetic susceptibility and environmental factors. In both of these cases there needs to be an increase in the production of insulin either by delivering insulin by intravenous or oral methods, or by taking medication. The following insulin ELISA kit will allow the determination of quantitative levels insulin that are present, thereby helping to determine what the correct dose of insulin is required in order to rectified the deficiency.
The minimum sensitivity detection limit of insulin using current human insulin ELISA kit was approximately 0.25 µIU/ml. The dynamic range for this assay is 0.25 – 200.0 µIU/ml.
- Evolution of insulin: from human to analog. Am J Med. (2014) 127 (10 Suppl): S25-38. Review. Tibaldi J.M.
- Insulin Signaling in the Control of Glucose and Lipid Homeostasis. Handb Exp Pharmacol. (2016) 233: 51-71. Review. Saltiel A.R.
- Insulin tactics in type 2 diabetes. Med Clin North Am. (2015) 99 (1): 157-86. Review. Meah F. and Juneja R.
- Full Name: Insulin ELISA Kit
- Reactivity: Human
- Sample Type: Serum, Plasma
- Sensitivity: 0.25 µIU/ml