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Glutamate ELISA Kit

INTENDED USE

Human glutamate ELISA kit can be used for measuring in vitro quantitative amounts of glutamate in human serum, plasma (EDTA) and urine. This assay has a sensitivity detection limit of 0.3 µg/ml.

BACKGROUND

Glutamate, in neuroscience is used to describe the anion of glutaminc acid in its functions as neurotransmitter that has the ability to send signals in the brain and throughout the nerves in the body. It is synthesised from glutamine using glutaminase enzyme as part of the glutamate-glutamine cycle within the central nervous system. This process usually occurs in the presynaptic neuron or even in the neighboring glial cells.

It is a major constituent of a large number of proteins and can also serve as a metabolic precursor for the neurotransmitter GABA, through the action of glutamate decarboxylase enzyme. Glutamate is vital for many bodily functions which includes sending messages for body movement, creation and the management of memory and regulating the human thought process. It is also very important during the development of the brain. Excess levels of glutamate in the brain have been linked with a number of neurological diseases for example Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, amyotrophic lateral sclerosis (ALS) and stroke. Also, difficulty in making or even using human glutamate have been associated to many mental health disorders such as obsessive-compulsive disorder (OCD), autism, depression and schizophrenia.

GLUTAMATE ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Adhesive Foil.
  • Reaction Plate.
  • Extraction Plate.
  • Wash Buffer Concentrate (50x).
  • Enzyme Conjugate.
  • Substrate (Chromogenic).
  • Stop Solution.
  • Glutamate Microtiter Strips.
  • Antiserum – Rabbit anti-glutamate antibody.
  • Assay Buffer.
  • Equalizing Reagent (Lyophilized).
  • Standards (A-F) and Controls (1+2) – 0-60 ng/ml.
  • D-Reagent – Crosslinking agent in dimethylsulfoxide.
  • Q-Buffer.
  • Diluent.
  • NaOH.

SENSITIVITY

The minimum sensitivity detection limit of human glutamate using current glutamate ELISA kit was approximately 0.3 µg/ml. The dynamic range for this assay is 0.6 – 60 µg/ml.

ASSAY CHARACTERISTICS

– Glutamate: ELISA
– Expected Reference Values: 5 – 100 µmol/g creatinine (Urine) and 2.2 – 30 µg/mL (Serum/Plasma).
– Analytical Sensitivity (Limit of Detection): 0.3 µg/Ml
– Analytical Specificity (Cross Reactivity): Glutamate (100%), Aspartate (0.09%), Glycine, Glutamine, 5-aminovaleric acid, Alanine (< 0.01%).
– Intra Assay Precision: 6.3 – 7.3%
– Inter Assay Precision: 13 – 19%
– Linearity: 83 – 111% (Urine) and 82 – 98% (Serum).
– Recovery: 83 – 105% (Urine) and 96 – 104% (Serum).

REFERENCES

  1. Maintaining the presynaptic glutamate supply for excitatory neurotransmission. J Neurosci Res. (2015) 93 (7): 1031-44. Review. Marx M.C., et al.
  2. Glutamate as a neurotransmitter in the healthy brain. J Neural Transm (Vienna). (2014) 121 (8): 799-817. Review. Zhou Y. and Danbolt N.C.
  3. The glutamate hypothesis in ALS: pathophysiology and drug development. Curr Med Chem. (2014) 21 (31): 3551-75. Review. Blasco H., et al.
  4. Glutamate: a truly functional amino acid. Amino Acids. (2013) 45 (3): 413-8. Review. Brosnan J.T. and Brosnan M.E.
  5. Multiple sclerosis and glutamate excitotoxicity. Rev Neurosci. (2013) 24 (1): 71-88. Review. Kostic M., et al.

ADDITIONAL INFORMATION

  • Full Name: Glutamate ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma (EDTA), Serum, Urine
  • Sensitivity: 0.3 µg/ml

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