GET A QUOTE

GBM Ab ELISA Kit

  • Created on the 12 June, 2017.

BACKGROUND

Glomerular basement membrane (GBM) is the basal lamina layer of glomerulus which can be found in the kidney. The GBM, glomerular capillary endothelial cells and filtration slits (between podocytes) all are vital in performing the function of filtrating of the glomerulus, this separates the blood present in the capillaries from the filtrate (formed in the Bowman’s capsule). The GBM is essentially a fusion of the podocyte basal laminas and the endothelial cell. The GBM is made up of three layers: the lamina rara externa which is adjacent to podocyte processes, lamina densa which is the dark central zone and the lamina rara interna which is adjacent to endothelial cells. The glomerular membrane is made up of mesangial cells and modified pericytes which can separate capillaries from each other in other parts of the body. The podocytes which are responsible for adjoining them contain filtration slits with a diameter 25nm (formed by pseudopodia). The pathological condition which have been associated with GBM include: Goodpasture’s syndrome (which can also be called anti-glomerular basement membrane disease), Nephrotic syndrome, diabetic glomerulosclerosis and Alport syndrome. ELISA assay have now been developed which consist of the corresponding pure antigen are able to display specificities and sensitivities up to 99%.

INTENDED USE

Human GBM ELISA kit can be used for in-vitro quantitative analysis of IgG autoantibodies against glomerular basement membrane (GBM) in human plasma and serum. This assay has a minimum analytical sensitivity limit of 1.0 U/ml.

SENSITIVITY

The minimum detection sensitivity level of human glomerular basement membrane (GBM) using this human GBM ELISA kit was 1.0 U/ml. The dynamic assay range for this kit is 20.0 – 200.0 U/ml.

REFERENCES

  1. Ultrastructure of developing kidney glomerular basement membranes: temporal changes in binding of anti-laminin IgG and cationized ferritin. Microsc Res Tech. (1994) 28 (2): 81-94. Review. Abrahamson D.R. and St John P.L.
  2. Organogenesis of the kidney glomerulus: focus on the glomerular basement membrane. Organogenesis. (2011) 7 (2): 75-82. Review. Miner J.H.
  3. Goodpasture’s syndrome: a clinical update. Autoimmun Rev. (2015) 14 (3): 246-53. Review. Greco A., et al.
  4. Concurrent anti-glomerular basement membrane disease and membranous glomerulonephritis: a case report and literature review. Clin Nephrol. (2006) 66 (2): 120-7. Review. Troxell M.L., et al.
  5. Diabetic nephropathy with interstitial nephritis presenting with a false-positive anti-GBM antibody. Clin Nephrol. (2002) 57 (5): 381-5. DeAangelo A.J., et al.

ADDITIONAL INFORMATION

  • Full Name: GBM Ab ELISA Kit
  • Reactivity: Human
  • Sample Type: Plasma, Serum
  • Sensitivity: 1.0 U/ml

OTHER RELATED ELISA KITS

TESTIMONIALS arrow icon

Your secretory IgA ELISA kit gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA kit performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA kits from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA kits. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford