CHV ELISA Kit (Canine Herpes Virus)

Full Name: CHV ELISA Kit (Canine Herpes Virus)
Reactivity: Canine
Sample Type: Serum, Plasma
Sensitivity: Qualitative

BACKGROUND

Canine herpes virus (CHV) is also commonly referred to as “fading puppy syndrome”, a virus that is a member of the Herpesviridae family which is often responsible for fatal hemorrhagic disease especially in puppies that are under 2-3 weeks old. Due to the fact that the thermos regulation of young pups does not function well, this can lead the virus multiplying rapidly and causing the pups to be heavily infected. Consequently, this infection will result in a high rate of mortality for many puppies. In adult dogs, this virus is predominately responsible for infecting the reproductive tract, this causes it to be passed to puppies during birth or even be sexually transmitted. This can lead to infertility, stillbirths and abortion.

Diagnosing this disease in puppies is frequently accomplished through necropsy and treating the affected puppies is often difficult, antibodies to CHV have been used to inject into the abdomen. Some of the important things to consider during the diagnosis of CHV include: looking at clinical history, clinical signs and analysing laboratory findings (such as antibody detection methods). Following assay is a monoclonal mediated antibody system which is specific in detecting antibodies against canine herpes virus in serum and plasma of dogs.

INTENDED USE

CHV ELISA kit is intended for measuring antibodies against canine herpes virus (CHV) in samples of plasma or serum.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Coated Microtiter Strips.
Strip Holder.
ELISA Buffer.
Negative Control (Freeze Dried).
Positive Control (Freeze Dried).
HRPO Conjugated Anti-Species Antibodies.
Wash Solution (200x Concentrated).
Substrate Buffer A.
Substrate Buffer B.
Stop Solution.
Plastic Cover Seal.

SENSITIVITY

The standard range of antibodies to canine herpes virus (CHV) using current CHV ELISA kit is qualitative and quantitative. Please contact us for the protocol insert for more details.

ASSAY CHARACTERISTICS

– Negative Control: OD ≤ 0.450 and an end point titer ≤ 30.
– Weak Positive Control: An extinction ≥ 0.900 OD units and an end point titer ≥ 90.
– End point titer of the sample is the dilution, which gives an extinction just above 0.250 OD units (450 nm).
– Sample Scored Positive: If OD is higher than 2.5 x OD of the negative control.

REFERENCES

Serodiagnosis of canine herpesvirus infection–development of an enzyme-linked immunosorbent assay and its comparison with two improved methods of serum neutralization test. Nihon Juigaku Zasshi. (1990) 52 (2): 241-50. Takumi A., et al.
Longitudinal study of viruses associated with canine infectious respiratory disease. J Clin Microbiol. (2004) 42 (10): 4524-9. Erles K., et al.
Risk factors and reproductive disorders associated with canine herpesvirus-1 (CHV-1). Theriogenology. (2004) 61 (4): 619-36. Ronsse V., et al.
Detection of high levels of canine herpes virus-1 neutralising antibody in kennel dogs using a novel serum neutralisation test. Res Vet Sci. (1999) 66 (3): 273-5. Reading M.J. and Field H.J.

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