Yersinia Enterocolitica IgM ELISA Kit

Full Name: Yersinia Enterocolitica IgM ELISA Kit
Reactivity: Human
Sample Type: Plasma, Serum
Sensitivity: 1.02 U/ml

BACKGROUND

Yersinia enterocolitica consists of 11 other species: Y. enterocolitica, Y. pseudotuberculosis, Y. kristensenii, Y. mollaretii, Y. aldovae, Y. intermedia, Y. frederiksenii, Y. bercovieri, Y. pestis, Y. ruckeri and Y. rohdei. It is member of the Enterobacteriaceae family and is a gram-negative bacterium which is bacillus shaped.

Y. enterocolitica can cause Yersiniosis disease and this is found to be an animal-borne disease which is usually present in human, pig, bird, cattle and deer. It causes symptoms such as; enterocolitis, acute diarrhea, pseudoappendicitis, terminal ileitis and mesenteric lymphadenitis. Colonoscopy, tube agglutination, stool culture analysis, computed tomography scanning imaging studies, radio-immunoassays, and enzyme-linked immunosorbent assays are techniques that have been utilised to research these infections.

INTENDED USE

Human Yersinia enterocolitica IgM ELISA kit is intended for measuring in-vitro concentrations of human IgM-class antibodies against Yersinia enterocolitica (Yersinia-IgM) using serum or plasma. This assay has a minimum analytical sensitivity limit of 1.02 U/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Yersinia enterocolitica antigen coated microtiter strips.
Enzyme Conjugate.
TMB Substrate.
Stop Solution.
Sample Diluent.
Washing Buffer (10x).
Control Positive.
Control Weak Positive.
Control Cut-Off.
Control Negative.

SENSITIVITY

The minimum detection sensitivity level of human IgM antibodies to Yersinia enterocolitica (Yersinia-IgM) using current Yersinia IgM ELISA kit was 1.02 U/ml. The dynamic range for this assay is 1.0 – 110.0 U/ml.

ASSAY CHARACTERISTICS

– Yersinia Enterocolitica ELISA: IgM
– Intra-Assay-Precision: 10.3 %
– Inter-Lot-Precision: 3.9 – 12.8 %
– Analytical Sensitivity: 1.02 U/mL
– Linearity: 66 – 114 %
– Recovery: 74 – 98 %
– Cross-Reactivity: No cross-reactivity to Helicobacter pylori.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, triglycerides up to 5.0 mg/mL and hemoglobin up to 8.0 mg/mL.
– Clinical Sensitivity: 100 %
– Clinical Specificity: 92 %

REFERENCES

Measurement of IgM and IgG antibodies to Yersinia by solid-phase radioimmunoassay. J Infect Dis. (1978) 138 (2): 232-6. Granfors K., et al.
Yersinia-specific antibodies in serum and synovial fluid in patients with Yersinia triggered reactive arthritis. Ann Rheum Dis. (1994) 53 (8): 535-9. Mäki-Ikola O., et al.

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