Yersinia Enterocolitica IgM ELISA Kit

INTENDED USE

Human Yersinia enterocolitica IgM ELISA kit is intended for measuring in-vitro concentrations of human IgM-class antibodies against Yersinia enterocolitica (Yersinia-IgM) using serum or plasma. This assay has a minimum analytical sensitivity limit of 1.02 U/ml.

BACKGROUND

Yersinia enterocolitica consists of 11 other species: Y. enterocolitica, Y. pseudotuberculosis, Y. kristensenii, Y. mollaretii, Y. aldovae, Y. intermedia, Y. frederiksenii, Y. bercovieri, Y. pestis, Y. ruckeri and Y. rohdei. It is member of the Enterobacteriaceae family and is a gram-negative bacterium which is bacillus shaped.

Y. enterocolitica can cause Yersiniosis disease and this is found to be an animal-borne disease which is usually present in human, pig, bird, cattle and deer. It causes symptoms such as; enterocolitis, acute diarrhea, pseudoappendicitis, terminal ileitis and mesenteric lymphadenitis. Colonoscopy, tube agglutination, stool culture analysis, computed tomography scanning imaging studies, radio-immunoassays, and enzyme-linked immunosorbent assays are techniques that have been utilised to research these infections.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Yersinia enterocolitica antigen coated microtiter strips.
• Enzyme Conjugate.
• TMB Substrate.
• Stop Solution.
• Sample Diluent.
• Washing Buffer (10x).
• Control Positive.
• Control Weak Positive.
• Control Cut-Off.
• Control Negative.

SENSITIVITY

The minimum detection sensitivity level of human IgM antibodies to Yersinia enterocolitica (Yersinia-IgM) using current Yersinia IgM ELISA kit was 1.02 U/ml. The dynamic range for this assay is 1.0 – 110.0 U/ml.

ASSAY CHARACTERISTICS

– Yersinia Enterocolitica ELISA: IgM
– Intra-Assay-Precision: 10.3 %
– Inter-Lot-Precision: 3.9 – 12.8 %
– Analytical Sensitivity: 1.02 U/mL
– Linearity: 66 – 114 %
– Recovery: 74 – 98 %
– Cross-Reactivity: No cross-reactivity to Helicobacter pylori.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, triglycerides up to 5.0 mg/mL and hemoglobin up to 8.0 mg/mL.
– Clinical Sensitivity: 100 %
– Clinical Specificity: 92 %

REFERENCES

1. Measurement of IgM and IgG antibodies to Yersinia by solid-phase radioimmunoassay. J Infect Dis. (1978) 138 (2): 232-6. Granfors K., et al.
2. Yersinia-specific antibodies in serum and synovial fluid in patients with Yersinia triggered reactive arthritis. Ann Rheum Dis. (1994) 53 (8): 535-9. Mäki-Ikola O., et al.

ADDITIONAL INFORMATION

• Full Name: Yersinia Enterocolitica IgM ELISA Kit
• Reactivity: Human
• Sample Type: Plasma, Serum
• Sensitivity: 1.02 U/ml

OTHER RELATED ELISA KITS

• Yersinia-IgG ELISA Kit
• Yersinia-IgA ELISA Kit
• Helicobacter Pylori-IgM ELISA Kit

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