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Yersinia Enterocolitica IgG ELISA Kit

INTENDED USE

Human Yersinia enterocolitica IgG ELISA kit is a protocol designed for analysing in-vitro quantitative amounts of human IgG antibodies to Yersinia enterocolitica (Yersinia-IgG) using samples of serum and plasma. This assay has a minimum analytical sensitivity limit of 1.92 U/ml.

BACKGROUND

Yersinia enterocolitica is a bacillus shaped, gram negative bacterium which belongs to the Enterobacteriaceae family. The genus Yersinia also is made of 11 other species Y. pseudotuberculosis, Y. intermedia, Y. enterocolitica, Y. rohdei, Y. aldovae, Y. bercovieri, Y. kristensenii, Y. mollaretii, Y. ruckeri, Y. pestis and Y. frederiksenii. Enterobacteriaceae have been identified to cause pseudoappendicitis, terminal ileitis, enterocolitis, mesenteric lymphadenitis and acute diarrhea, which can also lead to fatal sepsis if it is spread systemically. Yersiniosis disease can be caused by Y. enterocolitica infection, predominately an animal-borne disease occurring in humans, cattle, deer, pig and birds. Some of the common test which can be used to diagnose a Yersinia enterocolitica infection include; observing stool cultures, tube agglutination, joint aspiration in specific cases, colonoscopy, computed tomography scanning imaging studies, enzyme-linked immunosorbent assays and radioimmunoassays.

YERSINIA ENTEROCOLITICA IGG ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Yersinia enterocolitica antigen coated microtiter strips (IgG).
  • Calibrator Cut-Off.
  • Calibrator Negative.
  • Calibrator Positive.
  • Calibrator Weak Positive.
  • Sample Diluent.
  • Enzyme Conjugate.
  • TMB Substrate.
  • Stop Solution.
  • Washing Buffer (10x).

SENSITIVITY

The minimum detection sensitivity level of human IgG antibodies against Yersinia enterocolitica (Yersinia-IgG) using current Yersinia IgG ELISA kit was 1.92 U/ml. The dynamic range for this assay is 1.0 – 180.0 U/ml.

ASSAY CHARACTERISTICS

– Yersinia Enterocolitica ELISA: IgG
– Intra-Assay-Precision: 5.0 %
– Inter-Assay-Precision: 5.2 – 11.9 %
– Inter-Lot-Precision: 3.9 – 12.8 %
– Analytical Sensitivity: 1.92 U/mL
– Linearity: 70 – 113 %
– Recovery: 87 – 100 %
– Cross-Reactivity: No cross-reactivity to Helicobacter pylori.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, hemoglobin up to 8.0 mg/mL and triglycerides up to 5.0 mg/mL.
– Clinical Sensitivity: 100 %
– Clinical Specificity: 92 %

REFERENCES

  1. Serum immunoglobulin IgG subclass distribution of antibody responses to Yop proteins and lipopolysaccharide of Yersinia enterocolitica in patients with yersiniosis. Pol J Microbiol. (2007) 56 (4): 233-8. Rastawicki W. and Jakubczak A.
  2. High levels of IgG class antibodies to recombinant HSP60 kDa of Yersinia enterocolitica in sera of patients with uveitis. Br J Ophthalmol. (2004) 88 (2): 247-50. Cancino-Diaz J.C., et al.
  3. Yersinia antibodies in inflammatory joint diseases. Clin Exp Rheumatol. (1983) 1 (3): 215-8. Granfors K., et al.

ADDITIONAL INFORMATION

  • Full Name: Yersinia Enterocolitica IgG ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 1.92 U/ml

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