Vitamin H (Biotin) ELISA Kit


Vitamin H (biotin) ELISA kit is designed for detecting quantitative concentrations of biotin (vitamin H) in food. This assay has a minimum analytical sensitivity limit of 0.5 ng/ml.


Vitamin H, which is also more frequently called biotin, belongs to the family of B complex group of vitamins. These vitamins play essential roles in converting food (usually carbohydrates, amino acids and fats) into fuel (glucose) and this is used in order to produce energy. B vitamins are water soluble (i.e. the body is unable to store them), however, biotin can be produced by bacteria within the intestines and small amounts can be found in many foods. Some of the common foods include: cooked eggs, sardines, brewer’s yeast, nuts (pecans, almonds, walnuts and peanuts), soybeans, whole grains, banana, mushroom, cauliflower and other legumes (such as blackeye peas and beans).

The following vitamin H/biotin ELISA kit is intended for measuring quantitative levels of biotin in food. Many food-processing techniques have been found to destroy biotin, whereas less-processed versions of the same foods are found to contain more biotin. Also, raw egg whites are known to contain a protein called avidin and this protein is able to interfere with the ability of the body to absorb biotin. It is also recommended for strengthening nails and hair and is often found to be added to many cosmetic products for skin and hair. Biotin plays a vital role for normal embryonic growth and therefore is an important nutrient during pregnancy. When there is a lack of biotin in the body this results in nervous disorders, muscle pain, seborrhoea, dermatitis, tiredness and anorexia.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiter Plate: Coated with avidin.
  • Biotin Standards 1-6: Concentration 01, 2.5, 5, 10, 25 ng/ml.
  • Conjugate (Biotin-Alkaline-Phophatase).
  • Substrate Solution (PNPP).
  • Stop Solution (1 M NaOH).
  • Standard/Sample Diluent (PBS).
  • Washing Solution (PBS + Tween 20)(10x Concentrate).
  • Instruction Manual.


The minimum detection sensitivity level of vitamin H (biotin) using current vitamin H/biotin ELISA kit was 0.5 ng/ml. The standard range for this assay is 1.0 – 25.0 ng/ml.


– Vitamin H (Biotin): ELISA
– Sensitivity: 0.5ng/ml (based on the standard curve).
– Recovery: 98% based on spiked samples.
– Intra-Assay Precision: 3%


  1. Biotin: biochemical, physiological and clinical aspects. Subcell Biochem. (2012) 56: 1-19. Review. Said H.M.
  2. Vitamin H-regulated transgene expression in mammalian cells. Nucleic Acids Res. (2007) 35 (17): e116. Weber W., et al.
  3. A versatile Escherichia coli strain for identification of biotintransporters and for biotin quantification. Bioengineered. (2014) 5 (2): 129-32. Finkenwirth F., et al.
  4. Adequate intake of biotin in pregnancy: why bother? J Nutr. (2014) 144 (12): 1885-6. Mock D.M.
  5. Solid-phase synthesis of a biotin derivative and its application to the development of anti-biotin antibodies. Appl Biochem Biotechnol. (2010) 162 (1): 221-32. Papasarantos I., et al.


  • Full Name: Vitamin H (Biotin) ELISA Kit
  • Sample Type: Food
  • Sensitivity: 0.5 ng/ml



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