Vitamin H ELISA Kit (Biotin)

Full Name: Vitamin H ELISA Kit (Biotin)
Sample Type: Food
Sensitivity: 0.5 ng/ml

BACKGROUND

Vitamin H (biotin) belongs to the family of B complex group of vitamins. They function in helping to convert food (for example; carbohydrates, amino acids and fats) into fuel (e.g. glucose) that is used to produce energy. These are water soluble (i.e. the body is unable to store them), however, biotin can be produced by bacteria within the intestines and small amounts can be found in many foods. Some of the common foods include: cooked eggs, sardines, brewer’s yeast, nuts (pecans, almonds, walnuts and peanuts), soybeans, whole grains, banana, mushroom, cauliflower and other legumes (such as blackeye peas and beans).

The following assay is intended for measuring quantitative levels of biotin in food. Many food-processing techniques can destroy biotin, whereas less-processed versions of the same foods are found to contain more biotin. It is also recommended for strengthening nails and hair and is often found to be added to many cosmetic products for skin and hair.

INTENDED USE

Vitamin H ELISA kit is designed for detecting quantitative concentrations of biotin (vitamin H) in food. This assay has a minimum analytical sensitivity limit of 0.5 ng/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Microtiter Plate: Coated with avidin.
Biotin Standards 1-6: Concentration 01, 2.5, 5, 10, 25 ng/ml.
Conjugate (Biotin-Alkaline-Phophatase).
Substrate Solution (PNPP).
Stop Solution (1 M NaOH).
Standard/Sample Diluent (PBS).
Washing Solution (PBS + Tween 20)(10x Concentrate).
Instruction Manual.

SENSITIVITY

The minimum detection sensitivity level of vitamin H (biotin) using current vitamin H ELISA kit was 0.5 ng/ml. The standard range for this assay is 1.0 – 25.0 ng/ml.

ASSAY CHARACTERISTICS

– Sensitivity: 0.5ng/ml (based on the standard curve).
– Recovery: 98% based on spiked samples.
– Intra-Assay Precision: 3%

REFERENCES

Biotin: biochemical, physiological and clinical aspects. Subcell Biochem. (2012) 56: 1-19. Review. Said H.M.
Vitamin H regulated transgene expression in mammalian cells. Nucleic Acids Res. (2007) 35 (17): e116. Weber W., et al.
A versatile Escherichia coli strain for identification of biotintransporters and for biotin quantification. Bioengineered. (2014) 5 (2): 129-32. Finkenwirth F., et al.
Adequate intake of biotin in pregnancy: why bother? J Nutr. (2014) 144 (12): 1885-6. Mock D.M.
Solid-phase synthesis of a biotin derivative and its application to the development of anti biotin antibodies. Appl Biochem Biotechnol. (2010) 162 (1): 221-32. Papasarantos I., et al.

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