TRAIL ELISA Kit (TNF-Related Apoptosis-Inducing Ligand)


Human TRAIL ELISA kit is a procedure intended for analysing intended in vitro quantitative levels TNF-related apoptosis-inducing ligand (TRAIL) in human plasma, serum, saliva, cell culture supernatant and cell lysates. This assay has a minimum sensitivity limit of < 1.0 pg/ml.


TRAIL is composed of 281 amino acid sequence and possesses characteristics similar to a type II transmembrane protein. It can form homotrimer structures that have the ability to bind three receptor molecules. It can also bind to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII). It functions as a ligand in order to induce apoptosis and this process is caspase-8 dependent. This activates downstream effector caspases including; procaspase-3, -6 and -7, leading to activation of specific kinases. This whole process leads to transcription of genes which are known to antagonise the death signalling pathway and/or to promote inflammation. It has also been designated cluster of differentiation 253 (CD253) and tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10) – functioning as mediators of inflammatory responses and immune regulation.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human TRAIL antibody.
  • Standards: Concentrations 0, 15.6, 31.3, 62.5, 125.0, 250.0, 500, 1000 pg/ml, made up from lyophilized recombinant.
  • Sample Diluent Buffer
  • Biotinylated-Labelled Antibody
  • Antibody Diluent Buffer.
  • Avidin-Biotin-Peroxidase Complex (ABC).
  • ABC Diluent Buffer.
  • TMB Colour Developing Agent.
  • Stop Solution.


The minimum detection sensitivity level of TNF-related apoptosis-inducing ligand (TRAIL) using current TRAIL ELISA kit was 1.0 pg/ml. The dynamic range for this assay is 15.6 – 1,000 pg/ml.


– Specificity: Highly specific no detectable cross-reactivity with other cytokines.


  1. Onto better TRAILs for cancer treatment. Cell Death Differ. (2016) 23 (5): 733-47. Review. de Miguel D., et al.
  2. TRAIL-mediated signaling in prostate, bladder and renal cancer. Nat Rev Urol. (2011) 8 (8): 417-27. Review. Voelkel-Johnson C.
  3. The TRAIL of oncogenes to apoptosis. Biofactors. (2013) 39 (4): 343-54. Review. Oikonomou E. and Pintzas A.
  4. Regulation of TRAIL-receptor expression by the ubiquitin-proteasome system. Int J Mol Sci. (2014) 15 (10): 18557-73. Review. Sarhan D., et al.


  • Full Name: TRAIL ELISA Kit (TNF-Related Apoptosis-Inducing Ligand)
  • Reactivity: Human
  • Sample Type: Saliva, Serum, Cell Culture Supernatant, Plasma, Cell Lysates
  • Sensitivity: < 1.0 pg/ml



Online Enquiry Form

"*" indicates required fields

Please check mark information required:


Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford