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TNF-Related Apoptosis Inducing Ligand (TRAIL) ELISA Kit

INTENDED USE

Human TRAIL ELISA kit is a procedure intended for analysing intended in vitro quantitative levels TNF-related apoptosis-inducing ligand (TRAIL) in human plasma, serum, saliva, cell culture supernatant and cell lysates. This assay has a minimum sensitivity limit of < 1.0 pg/ml.

BACKGROUND

TRAIL is composed of 281 amino acid sequence and possesses characteristics similar to a type II transmembrane protein. The C-terminal extracellular domain is found to be conserved and can also be proteolytically cleaved from the cell surface, whereas the N-terminal cytoplasmic domain is not conserved across family members. TRAIL can form homotrimer structures that have the ability to bind three receptor molecules. TRAIL can bind to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII).

TRAIL is essentially a protein which functions as a ligand in order to induce apoptosis and this process is caspase-8 dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. This whole process leads to transcription of genes which are known to antagonise the death signalling pathway and/or to promote inflammation.

TRAIL has also been designated cluster of differentiation 253 (CD253) and tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10) – functioning as mediators of inflammatory responses and immune regulation.

TRAIL ELISA KIT CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-human TRAIL antibody.
  • Standards: Concentrations 0, 15.6, 31.3, 62.5, 125.0, 250.0, 500, 1000 pg/ml, made up from lyophilized recombinant human TNF-related apoptosis-inducing ligand.
  • Sample Diluent Buffer
  • Biotinylated TRAIL antibody
  • Antibody Diluent Buffer.
  • Avidin-Biotin-Peroxidase Complex (ABC).
  • ABC Diluent Buffer.
  • TMB Colour Developing Agent.
  • Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of TNF-related apoptosis-inducing ligand (TRAIL) using current human TRAIL ELISA kit was 1.0 pg/ml. The dynamic range for this assay is 15.6 – 1,000 pg/ml.

ASSAY CHARACTERISTICS

– TNF-Related Apoptosis-Inducing Ligand (TRAIL): ELISA
– Specificity: Highly specific no detectable cross-reactivity with other cytokines.

REFERENCES

  1. Onto better TRAILs for cancer treatment. Cell Death Differ. (2016) 23 (5): 733-47. Review. de Miguel D., et al.
  2. Tumor-necrosis-factor-related apoptosis-inducing ligand (TRAIL). Adv Exp Med Biol. (2014) 818: 167-80. Review. Fulda S.
  3. TRAIL-mediated signaling in prostate, bladder and renal cancer. Nat Rev Urol. (2011) 8 (8): 417-27. Review. Voelkel-Johnson C.
  4. The TRAIL of oncogenes to apoptosis. Biofactors. (2013) 39 (4): 343-54. Review. Oikonomou E. and Pintzas A.
  5. Regulation of TRAIL-receptor expression by the ubiquitin-proteasome system. Int J Mol Sci. (2014) 15 (10): 18557-73. Review. Sarhan D., et al.
  6. TRAIL-based therapeutic approaches for the treatment of pediatric malignancies. Curr Med Chem. (2013) 20 (17): 2254-71. Review. Gasparini C., et al.

ADDITIONAL INFORMATION

  • Full Name: TNF-Related Apoptosis-Inducing Ligand (TRAIL) ELISA Kit
  • Reactivity: Human
  • Sample Type: Saliva, Serum, Cell Culture Supernatant, Plasma, Cell Lysates
  • Sensitivity: < 1.0 pg/ml

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