TRAIL ELISA Kit (TNF-Related Apoptosis-Inducing Ligand)

INTENDED USE

Human TRAIL ELISA kit is a procedure intended for analysing intended in vitro quantitative levels TNF-related apoptosis-inducing ligand (TRAIL) in human plasma, serum, saliva, cell culture supernatant and cell lysates. This assay has a minimum sensitivity limit of < 1.0 pg/ml.

BACKGROUND

TRAIL is composed of 281 amino acid sequence and possesses characteristics similar to a type II transmembrane protein. It can form homotrimer structures that have the ability to bind three receptor molecules. It can also bind to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII). It functions as a ligand in order to induce apoptosis and this process is caspase-8 dependent. This activates downstream effector caspases including; procaspase-3, -6 and -7, leading to activation of specific kinases. This whole process leads to transcription of genes which are known to antagonise the death signalling pathway and/or to promote inflammation. It has also been designated cluster of differentiation 253 (CD253) and tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10) – functioning as mediators of inflammatory responses and immune regulation.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-human TRAIL antibody.
• Standards: Concentrations 0, 15.6, 31.3, 62.5, 125.0, 250.0, 500, 1000 pg/ml, made up from lyophilized recombinant.
• Sample Diluent Buffer
• Biotinylated-Labelled Antibody
• Antibody Diluent Buffer.
• Avidin-Biotin-Peroxidase Complex (ABC).
• ABC Diluent Buffer.
• TMB Colour Developing Agent.
• Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of TNF-related apoptosis-inducing ligand (TRAIL) using current TRAIL ELISA kit was 1.0 pg/ml. The dynamic range for this assay is 15.6 – 1,000 pg/ml.

ASSAY CHARACTERISTICS

– Specificity: Highly specific no detectable cross-reactivity with other cytokines.

REFERENCES

1. Onto better TRAILs for cancer treatment. Cell Death Differ. (2016) 23 (5): 733-47. Review. de Miguel D., et al.
2. TRAIL-mediated signaling in prostate, bladder and renal cancer. Nat Rev Urol. (2011) 8 (8): 417-27. Review. Voelkel-Johnson C.
3. The TRAIL of oncogenes to apoptosis. Biofactors. (2013) 39 (4): 343-54. Review. Oikonomou E. and Pintzas A.
4. Regulation of TRAIL-receptor expression by the ubiquitin-proteasome system. Int J Mol Sci. (2014) 15 (10): 18557-73. Review. Sarhan D., et al.

ADDITIONAL INFORMATION

• Full Name: TRAIL ELISA Kit (TNF-Related Apoptosis-Inducing Ligand)
• Reactivity: Human
• Sample Type: Saliva, Serum, Cell Culture Supernatant, Plasma, Cell Lysates
• Sensitivity: < 1.0 pg/ml

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