Testosterone ELISA Kit


Human testosterone ELISA kit is a reliable quantitative procedure for measuring testosterone in human serum and plasma. This assay has a minimum sensitivity detection limit of 0.08 ng/ml.


Testosterone (also called 17beta-hydroxy-4-androstene-3-one) is the most effective natural hormone which belong to the androgens family, it is composed of a C19 steroid which contains a keto and hydroxyl groups at the three and seventeen positions respectively and it has a molecular mass of 288.47g/mol. It is one of the most vital androgen that is secreted into the blood.

During circulating, the major part of testosterone known to be bound to plasma proteins such as sex hormone binding globuline (SHBG) and albumin. The major function is in the development of secondary male sex characteristics and its measurements can be helpful in evaluating the hypogonadal states. In males it can be secreted by Leydig cells of the testes and in females more than half of circulating testosterone is derived from peripheral conversion of androstenedione and the remainder is from the ovary and the adrenal glands.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Microtiterwells: Wells coated with a monoclonal anti-testosterone antibody.
  • Standard 0-6: Concentration 0, 0.2, 0.5, 1.0, 2.0, 6.0, 16 ng/ml.
  • Enzyme Conjugate: Consisting of antibody conjugated to horseradish peroxidase (HRP).
  • Substrate Solution: Tetramethylbenzidine (TMB).
  • Stop Solution.
  • Wash Solution (40x Concentrated).


The minimum sensitivity detection limit of testosterone using current testosterone ELISA kit was approximately 0.08 ng/ml. The dynamic range for this assay is 0.2 – 16.0 ng/ml.


– Assay Dynamic Range: 0.083 ‒ 16.0 ng/mL.
– Specificity (Cross Reactivity): Testosterone (100.0%), DHT (12.9%), 11β-Hydroxyestosterone (3.3%), 19-Nortestosterone (3.3%), Androstenedione (0.9%), 5α-Dihydrotestosterone (0.8%), DHEA (0.3%), 17α-Methyltestosterone (0.1%), DHEA-S, Epitestosterone, Progesterone, Cortisol, Estrone, Estradiol, Estriol, Danazol (< 0.1%).
– Analytical Sensitivity: 0.083 ng/mL
– Intra-Assay Variability: 3.3 – 4.2%
– Inter-Assay Variability: 4.7 – 9.9%
– Recovery: 86.9 – 110.7%
– Linearity: 86.1 – 110.6%
– Drug Interferences: None
– Interfering Substances: None


  1. Testosterone: a vascular hormone in health and disease. J Endocrinol. (2013) 217 (3): R47-71. Review. Kelly D.M. and Jones T.H.
  2. Testosterone-Therapy and Prostate Cancer. Urol Clin North Am. (2016) 43 (2): 209-16. Review. Davidson E. and Morgentaler A.
  3. Testosterone Replacement Therapy and the Cardiovascular System. Curr Atheroscler Rep. (2016) 18 (4): 19. Review. Naderi S.


  • Full Name: Testosterone ELISA Kit
  • Reactivity: Human
  • Sample Type: Serum, Plasma
  • Sensitivity: 0.08 ng/ml


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