SS-A 60 Ab ELISA Kit (Sjögren Syndrome Type A Antigen 60)

Full Name: SS-A 60 Ab ELISA Kit (Sjögren Syndrome Type A Antigen 60)
Reactivity: Human
Sample Type: Plasma, Serum
Sensitivity: 0.5 U/ml


Anti-SS-A 60kDa antibodies are found in approximately 70% of Sjögren’s patients. They are associated with earlier disease onset, more extraglandular manifestations, and pulmonary inflammation. SS-A 60kDa antibodies cross the placenta and can cause neonatal lupus syndromes like congenital heart block.

Ro60 is a 60kDa protein that localizes to the nucleus and cytoplasm. It contains RNA recognition motifs and binds small cytoplasmic Y RNAs. Ro60 interacts with Ro52 and La to form stabile ribonucleoprotein particles. Although its function is not fully clear, Ro60 is thought to be involved in quality control of misfolded noncoding RNAs.

The pathogenesis of anti-Ro60 autoimmunity is not fully understood. Apoptotic blebs containing modified Ro60 antigens on salivary gland epithelial cells may trigger autoantibody production. Anti-Ro60 antibodies could promote inflammation through macrophage activation and interferon signaling. They may also directly impair exocrine gland function.


Human SS-A 60 Ab ELISA kit can be used for detecting in-vitro quantitative levels of IgG autoantibodies to Sjögren syndrome type A antigen 60 (SS-A 60 antibody, anti-SS-A 60, anti-Ro 60, SS-A 60 Ab) in human serum or plasma. This assay has a minimum analytical sensitivity limit of 0.5 U/ml.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Divisible Microplate: Consisting of 12 modules of 8 wells, highly purified SS-A 60 antibodies bound to microwells.
  • Calibrators (A-F): Concentration 0-200 U/ml, made up of SS-A 60.
  • Controls Positive And Control Negative: Containing human anti-SS-A 60 in serum/buffer matrix.
  • Sample Buffer (5x Concentrated).
  • Enzyme Conjugate: Contains anti-human IgG antibodies, HRP labelled.
  • TMB Substrate.
  • Stop Solution: Contains acid.
  • Wash Buffer (50x Concentrated).
  • Instruction Manual.
  • Certificate of Analysis.


The minimum detection sensitivity level of human Sjögren syndrome type A antigen 60 (anti-SS-A 60, SS-A 60 Ab, anti-Ro 60) IgG autoantibodies using this SS-A 60 Ab ELISA kit was 0.5 U/ml. The dynamic range for this assay is 12.5 – 200.0 U/ml.


– Results Interpretation: Normal < 15 U/ml, Cut-Off 15-25 U/ml, Elevated ≥ 25 U/ml.
– Specificity: Highly specific to autoantibodies against SS-A 60, no cross reaction to ENA antigens was displayed.
– Sensitivity: 0.5 U/ml.
– Intra Assay: 2.7 – 3.2%
– Inter Assay: 1.8 – 6.3%
– Interference: None.


  1. Ro(SS-A) and anti-Ro(SS-A): an update. Rev Rhum Engl Ed. (1998) 65 (1): 45-57. Review. Sibilia J.
  2. Laboratory evaluation of patients with Sjögren’s syndrome. Clin Biochem. (1992) 25 (3): 213-22. Review. Fox R.I., et al.
  3. Neonatal lupus syndromes. Curr Opin Rheumatol. (1994) 6 (5): 523-9. Review. Buyon J.P.
  4. Anti-Ro/SS-A and anti-La/SS-B antibodies associated with cardiac involvement in childhood systemic lupus erythematosus. Ann Rheum Dis. (1997) 56 (4): 272-4. Oshiro A.C., et al.
  5. Anti-Ro/SSA and La/SSB antibodies. Autoimmunity. (2005) 38 (1): 55-63. Review. Franceschini F. and Cavazzana I.


Online Enquiry Form

"*" indicates required fields

Please check mark information required:


Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford