Sirtuin 1 ELISA Kit (SIRT1)

Full Name: Sirtuin 1 ELISA Kit (SIRT1)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 0.188 ng/ml


SIRT1, known as sirtuin 1, is a NAD-dependent deacetylase, SIRT1 removes acetyl groups from target proteins to modulate their activation states in response to metabolic or environmental signals. The longevity and health-related effects of calorie restriction relate partly to SIRT1 activity enhancement.

SIRT1 deacetylates histones in chromosomal DNA as well as various transcription factors and co-regulators to influence gene expression patterns. This alters balances between anabolism and catabolism, oxidative stress responses, apoptosis signaling, and circadian behaviors in cells. For instance, SIRT1 enhances fat mobilization, inhibits fat storage/growth pathways, stimulates mitochondrial biogenesis, reduces inflammation through transcription factor regulation, and beneficially impacts circadian clock maintenance – effects amplified by nutrient deprivation.

Such SIRT1 regulatory impacts likely contribute to improved health and survival observed with calorie restriction in some species. Boosting SIRT1 activity pharmacologically aims to mimic such effects. Compound screening reveals small molecule sirtuin activating compounds (STACs) can enhance SIRT1 activity to realize therapeutic effects in metabolic, neurodegenerative, inflammatory, and aging-related disease models. However, designing isoform-selective SIRT1 modulators has proved challenging. Off-target effects and incomplete understanding of downstream mechanisms still require further study.


Human sirtuin 1 ELISA kit can measure concentrations of SIRT1 (sirtuin 1, SIR2-like protein 1, homolg 1, EC 3.5.1, SIR2alpha) present is plasma, serum, biological fluids or tissue homogenate samples.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti- Sirtuin 1.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.


For this sirtuin 1 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.312, 0.625, 1.25, 2.5, 5, 10, 20 ng/ml.
Reactivity: Human
Sensitivity: 0.188 ng/ml
Range: 0.313 – 20 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.


– Specificity: Highly specific for SIRT1, no cross reactivity or interference between SIRT1 and analogues was detected.
– Recovery: Serum (86 – 105%), EDTA Plasma (90 – 104%), Heparin Plasma (86 – 104%).
– Linearity: Serum (86 – 98%), EDTA Plasma (82 – 100%), Heparin Plasma (82 – 98%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%


  1. SIRT1 and aging related signaling pathways. Mech Ageing Dev. (2020) 187: 111215. Chen C., et al.
  2. Sirtuin 1 (SIRT1) and steroid hormone receptor activity in cancer. J Endocrinol. (2012) 213 (1): 37-48. Moore R.L., et al.
  3. The Beneficial Roles of SIRT1 in Neuroinflammation-Related Diseases. Oxid Med Cell Longev. (2020) 2020: 6782872. Jiao F. and Gong Z.
  4. Sirtuin 1 and Alzheimer’s disease: An up-to-date review. Neuropeptides. (2018) 71: 54-60. Rizzi L. and Roriz-Cruz M.


Online Enquiry Form

"*" indicates required fields

Please check mark information required:


Your secretory IgA ELISA gave good results and I was also really impressed with how quickly we received it.

L. Johnston
PhD Student / University of Glasgow

It is refreshing to know that you have a technical team that is very knowledgeable. I have already recommended your company to other researchers in our department.

Dr. P. Anderson
Lecturer / University College London (UCL)

I am a first time user and found that your instruction manual was very easy to follow. The insulin ELISA assay performed well and I was happy with the results that were generated.

J. Thomas
Senior Technician / Addenbrooke’s Hospital

I carried out a pilot study comparing the performance of many ELISA assay's from different suppliers and found your kits to be one of the better performers. We observed good linearity and tight replicates.

Dr. C. Davies
Lead Scientists / AstraZeneca

You are my first point of contact when I am looking to purchase ELISA. You have such an easy and simple system, yet it is very effective.

A. Shaw
Purchasing / University of Oxford