Reelin ELISA Kit (RELN)

Full Name: Reelin ELISA Kit (RELN)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 9.375 pg/ml

INTRODUCTION

Reelin, a glycoprotein found in the matrix plays a role, in the proper migration and positioning of neurons during brain development. When there are mutations in the gene it can lead to organization of the cortex and cognitive difficulties. Cajal Retzius cells located in the zone of the developing cortex secrete reelin. This protein then binds to receptors on migrating neurons triggering a series of signals that promote neuron movement and adhesion. Throughout corticogenesis it establishes gradients that guide migration of neurons from the zone to the cortical plate. Additionally reelin signaling helps stabilize leading processes of migrating neurons to prevent migration.

Apart from its role in migration Reelin also regulates plasticity and dendritic shape in the postnatal brain. It contributes to stabilizing spines and enhancing long term potentiation (LTP) a process for learning and memory. By stimulating clustering of ApoER2 and VLDLR receptors within densities reelin promotes maturation of synapses. Moreover it aids in transporting NMDAR and AMPAR receptors during plasticity.

In experiments with mice that had altered reelin cognitive problems were resolved when they were treated with reelin-peptides. By enhancing the effects on plasticity and stability it may be possible to counteract abnormalities that affect learning and memory. However manipulating the nature of the signaling system poses challenges.

INTENDED USE

Human Reelin ELISA kit can measure concentrations of RELN (Reelin, RL, Reeler) present is plasma, serum, biological fluids or tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-RELN.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this Reelin ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml.
Reactivity: Human
Sensitivity: 9.375 pg/ml
Range: 15.625 – 1000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for RELN, no cross reactivity or interference between RELN and analogues was detected.
– Recovery: Serum (88 – 101%), EDTA Plasma (88 – 104%), Heparin Plasma (89 – 104%).
– Linearity: Serum (91 – 103%), EDTA Plasma (83 – 99%), Heparin Plasma (80 – 99%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. Reelin Functions, Mechanisms of Action and Signaling Pathways During Brain Development and Maturation. Biomolecules. (2020) 10 (6): 964. Jossin Y.
2. The risks of RELN polymorphisms and its expression in the development of otosclerosis. PLoS One. (2022) 17 (6): e0269558. Priyadarshi S., et al.
3. How does Reelin signaling regulate the neuronal cytoskeleton during migration? Neurogenesis (Austin). (2016) 3 (1): e1242455. Chai X. and Frotscher M.
4. Heterozygous missense mutation of the RELN gene is one of the causes of epilepsy. Neurol Res. (2022) 44 (3): 262-267. Fang X.Q., et al.

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