SARS-CoV-2 S1 IgM ELISA Kit

Full Name: SARS-CoV-2 S1 IgM ELISA Kit
Reactivity: Human
Sample Type: Plasma, Serum
Sensitivity: Qualitative

INTRODUCTION

SARS-CoV-2 was responsible for causing coronavirus disease 2019 (COVID-19) pandemic. Symptoms from this virus include; pneumonia, vomiting, loss of smell and taste, headaches, stroke, nerve pain, seizures, dizziness, nausea and acute respiratory distress. syndrome. It can be transmitted during an outbreak through inhalation, which can be through close contact with bodily fluids and/or aerosolized droplets. The virus may also enter the body via ingestion with contaminated food or water. This disease is not contagious until after symptoms appear.

SARS-CoV may also be transmitted by close contact with an infected person or animal. Symptoms typically develop 2–3 days after exposure to the virus with a maximum symptom duration of about 1 week. The disease is diagnosed by a combination of clinical symptoms, virus isolation and PCR on respiratory specimens. SARS-CoV may also be transmitted by close contact with an infected person or animal.

Deleted: The spike protein is essentially required during viral infection and pathogenesis processes. There are two sub-units S1 and S2. The S1 consists of the receptor binding domain (RBD) that can recognise and bind to host receptors and the S2 is responsible for mediating the viral cell membrane fusion process. The RBD is a typical three-finger protein domain found in many spike proteins. The two N-termini of the S1 and S2 are connected by an intracellular C-terminal tail, which forms a flexible loop when bound to receptors. During receptor binding, this loop undergoes a conformational change that allows for host cell fusion. For example, if an host cell membrane is exposed at the junction between two cells, the RBD triggers receptor binding and host cell fusion at this site.

INTENDED USE

Human SARS-CoV-2 S1 IgM ELISA kit is designed for analysing amounts of anti-SARS-CoV-2 spike protein S1 protein (S1)(IgM class antibodies) in samples of serum or plasma.

CONTENT

Each kit contains the following components and is sufficient for 96 tests.

One Aluminium Pouch: Containing microwell plate bound with SARS-CoV-2 S1 protein.
100x Detection Antibody Solution: HRP coated anti IgM monoclonal antibody.
10x Standard.
10x Wash Buffer.
5x Assay Buffer.
Substrate Solution.
Stop Solution.

TYPICAL RESULTS

Serum From Healthy Patients: 0.116, 0.203, 0.148, 0.102, 0.072, 0.155, 0.08, 0.197, 0.064, 0.092 (OD450).
Serum From COVID-19 Patients: 1.404, 0.965, 0.675, 0.358, 1.148, 1.353, 0.293, 0.88, 1.591, 3.814 (OD450).

It is advised that each laboratory establishes its own normal and pathological refence range for detection of anti-S1 IgM antibodies.

ASSAY CHARACTERISTICS

– Inter Assay Precision: CV = Less than 10%
– Intra Assay Precision: CV = Less than 8%.

REFERENCES

Assessment of S1-, S2-, and NCP-Specific IgM, IgA, and IgG Antibody Kinetics in Acute SARS-CoV-2 Infection by a Microarray and Twelve Other Immunoassays. J Clin Microbiol. (2021) 59 (5): e02890-20. Semmler G., et al.
SARS-CoV-2 Spike Protein Extrapolation for COVID Diagnosis and Vaccine Development. Front Mol Biosci. (2021) 8: 607886. Malik Y.S., et al.
Lack of cross-reactivity between rheumatoid factor IgM and anti-S1 receptor binding domain of SARS-CoV-2 IgM: a case-control study. Clin Exp Rheumatol. (2022) 40 (7): 1417-1419. Pellegrino G., et al.
SARS-CoV-2 S1 and N-based serological assays reveal rapid seroconversion and induction of specific antibody response in COVID-19 patients. Sci Rep. (2020) 10 (1): 16561. Algaissi A., et al.

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