Ribonuclease T2 ELISA Kit (RNASET2)

INTENDED USE

Human ribonuclease T2 ELISA kit can measure concentrations of RNASET2 (ribonuclease T2, RNASE6PL, ribonuclease 6) present is plasma, serum, biological fluids or tissue homogenate samples. This assay has a minimum sensitivity detection limit of 0.188 ng/ml.

INTRODUCTION

RNASET2 refers to a ubiquitous lysosomal ribonuclease belonging to the T2 family involved in RNA catabolism and associated with diverse pathologies including cancer and neurological disorders. As the only RNASET enzyme localized within lysosomes, RNASET2 plays a vital role in cellular RNA turnover and maintenance of RNA homeostasis. However dysregulation compromises degradation capacity, enabling accumulation or loss of essential RNA species that alter subsequent gene expression patterns to drive disease emergence.

As part of the regulated RNA decay machinery, RNASET2 catalyzes hydrolysis of RNA internal phosphodiesteric linkages, generating 3’-monophosphorylated products following endonucleolytic cleavage. This initial breakdown of polymerized RNA then allows further degradation by other RNAses and phosphatases in successive steps towards nucleotide monomer recycling or clearance. Such cellular RNA catabolism proceeds at slower rates than cytoplasmic turnover pathways, operating instead as more persistent lysosomal mechanism activated by autophagy that collects RNA material over time.

Given such crucial involvement in RNA homeostasis especially for long-lived species, deficiencies in RNASET2 markedly alter neuronal RNA degradation capacity implicated in certain neurodegenerative conditions like Alzheimer’s and Parkinson’s diseases. Additionally, deregulated RNASET2-associates with tumor pathogenesis and chemotherapy resistance in ovarian, colorectal and melanoma cancers possibly tied to lysosomal dysfunction enabling malignant transcriptional patterns. Looking ahead, strategies that fine tune RNASET2 expression or localize its catalytic activity show promise for ameliorating pathogenesis rooted in RNA dyshomeostasis across cancer and chronic neurodegeneration settings.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-RNASET2 antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this RNASET2 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.312, 0.625, 1.25, 2.5, 5, 10, 20 ng/ml.
Reactivity: Human
Sensitivity: 0.188 ng/ml
Range: 0.313 – 20 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for RNASET2, no cross reactivity or interference between RNASET2 and analogues was detected.
– Recovery: Serum (94 – 102%), EDTA Plasma (91 – 103%), Heparin Plasma (86 – 102%).
– Linearity: Serum (85 – 103%), EDTA Plasma (84 – 95%), Heparin Plasma (80 – 98%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. Ribonuclease T2 from Aspergillus fumigatus promotes T helper type 2 responses through M2 polarization of macrophages. Int J Mol Med. (2020) 46 (2): 718-728. Chen J.J., et al.
2. Stress-induced RNASET2 overexpression mediates melanocyte apoptosis via the TRAF2 pathway in vitro. Cell Death Dis. 2014 Jan 23;5(1):e1022. Wang Q., et al.
3. T2 Family ribonucleases: ancient enzymes with diverse roles. Trends Biochem Sci. (2010) 35 (5): 253-9. Luhtala N. and Parker R.
4. Downregulation of tumor suppressor gene ribonuclease T2 and gametogenetin binding protein 2 is associated with drug resistance in ovarian cancer. Oncol Rep. (2014) 32 (1): 362-72. Yin F., et al.

ADDITIONAL INFORMATION

Full Name: Ribonuclease T2 ELISA Kit (RNASET2)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 0.188 ng/ml

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