OGG1 ELISA Kit (N-Glycosylase/DNA Lyase)
INTENDED USE
Human OGG1 ELISA kit can measure concentrations of N-glycosylase (OGG1, 8-hydroxyguanine, DNA lyase, 8-oxoguanine DNA glycosylase, AP lyase) present is plasma, serum, biological fluids or tissue homogenate samples.
INTRODUCTION
OGG1 (8-Oxoguanine DNA glycosylase) constitutes a DNA repair enzyme that removes oxidatively damaged guanine bases, initiating base excision repair to maintain genomic integrity. As one of the chief DNA lesions generated by reactive oxygen species, 8-oxo-7,8-dihydroguanine (8-oxoG) potently pairs with adenine instead of cytosine, causing G:C to T:A transversion mutations upon cellular replication. By recognizing and eliminating such mutagenic bases, OGG1 preserves fidelity of DNA polymerases against oxidative threats. Beyond canonical repair functions however, OGG1 exhibits intriguing DNA demethylation activity tied to epigenetic regulation in development and disease.
As a bifunctional DNA glycosylase, OGG1 directly removes aberrant 8-oxoG bases including opposing cytosines at sites of helix distortion via glycosylase and beta-lyase activities respectively. This generates an abasic apurinic/apyrimidic site ultimately replaced correctly by base excision repair machinery restoring intact base pairing. Thereby, OGG1 mitigates genomic instability and downstream defects driven by unchecked oxidative DNA damage that may otherwise trigger apoptosis or malignancy upon mutational accumulation.
Intriguingly, accumulating evidence also finds OGG1 catalyzes removal of 5-methylcytosines epigenetically modifying gene expression. This may enable active demethylation supporting chromatin regulation of key developmental genes and plasticity mechanisms implicated in learning. However overexpression also associates with various cancers, highlighting the precarious balance between advantageous and pathological consequences of OGG1 activity at both genetic and epigenetic levels. Evaluating expression levels by enzyme-linked immunosorbent assay may help gauge disease states and immune health.
CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- One 96-Well Plate: Pre-coated with anti-OGG1 antibody.
- Standards: Lyophilized recombinant.
- Sample/Standard Dilution Buffer.
- Biotinylated-labelled Antibody.
- Antibody Dilution Buffer.
- HRP-Streptavidin Conjugate (SABC).
- SABC Dilution Buffer.
- TMB Substrate.
- Wash Buffer (25x).
- Plate Sealer.
- Product Instructions.
TYPICAL RESULTS
For this OGG1 ELISA kit, it is recommended that a standard curve is generated for each assay carried out.
Standard Curve: 0, 0.156, 0.312, 0.625, 1.25, 2.5, 5, 10 ng/ml.
Reactivity: Human
Sensitivity: 0.94 ng/ml
Range: 0.156 – 10 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.
ASSAY CHARACTERISTICS
– Specificity: Highly specific for OGG1, no cross reactivity or interference between OGG1 and analogues was detected.
– Recovery: Serum (93 – 103%), EDTA Plasma (86 – 105%), Heparin Plasma (91 – 104%).
– Linearity: Serum (86 – 103%), EDTA Plasma (86 – 101%), Heparin Plasma (86 – 92%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%
REFERENCES
- An active alternative splicing isoform of human mitochondrial 8-oxoguanine DNA glycosylase (OGG1). Genes Environ. (2015) 37: 21. Furihata C.
- OGG1 in the Kidney: Beyond Base Excision Repair. Oxid Med Cell Longev. (2022) 2022: 5774641. Zhao F., et al.
- Roles of DNA repair enzyme OGG1 in innate immunity and its significance for lung cancer. Pharmacol Ther. (2019) 194: 59-72. Vlahopoulos S., et al.
- The OGG1 gene encodes a repair enzyme for oxidatively damaged DNA and is involved in human carcinogenesis. Antioxid Redox Signal. (2001) 3 (4): 597-609. Shinmura K. and Yokota J.
ADDITIONAL INFORMATION
- Full Name: OGG1 ELISA Kit (N-Glycosylase/DNA Lyase)
- Reactivity: Human
- Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
- Sensitivity: 0.094 ng/ml
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