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MSTN ELISA Kit (MSTN/Growth Differentiation Factor 8)

Full Name: MSTN ELISA Kit (MSTN/Growth Differentiation Factor 8)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 18.75 pg/ml

INTRODUCTION

Myostatin, also known as Growth Differentiation Factor 8 (GDF 8) is a protein that belongs to the transforming growth factor β family. Its role is to limit the growth of muscles by influencing the differentiation of cells during development and the growth of muscle cells, in adulthood. Myostatin works by signaling through activin receptors and acting as a regulator of muscle growth. In mammals the gene responsible for myostatin production, MSTN is relatively stable. Encodes a protein made up of 375 amino acids.

Scientific studies have indicated that levels of GDF8 could indicate the severity of heart muscle damage during a infarction (AMI) similar to peak troponin I levels. This implies that GDF8 might serve as an indicator of severity after AMI. Myostatin has been observed to inhibit muscle growth and restrict the proliferation of mesenchymal stem cells (MSCs). It has also been found to influence components of the Wnt pathway and suppress Wnt4 expression, which stimulates MSC proliferation. The complexity in how myostatin transmits signals arises from its involvement with signaling pathways and it is through their precise integration that myostatin exerts its inhibitory effects, on growth.

INTENDED USE

Human MSTN ELISA kit can measure concentrations of MSTN/Growth Differentiation Factor 8) (MSTN) present is plasma, serum, biological fluids or tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • One 96-Well Plate: Pre-coated with anti-MSTN antibody.
  • Standards: Lyophilized recombinant.
  • Sample/Standard Dilution Buffer.
  • Biotinylated-labelled Antibody.
  • Antibody Dilution Buffer.
  • HRP-Streptavidin Conjugate (SABC).
  • SABC Dilution Buffer.
  • TMB Substrate.
  • Wash Buffer (25x).
  • Plate Sealer.
  • Product Instructions.

TYPICAL RESULTS

For this MSTN ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 pg/ml.
Reactivity: Human
Sensitivity: 18.75 pg/ml
Range: 31.25 – 2000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for MSTN, no cross reactivity or interference between MSTN and analogues was detected.
– Recovery: Serum (85 – 102%), EDTA Plasma (86 – 105%), Heparin Plasma (87 – 105%).
– Linearity: Serum (85 – 95%), EDTA Plasma (90 – 98%), Heparin Plasma (83 – 99%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

  1. MSTN modification in goat mediated by TALENs and performance analysis. Chuan. (2022) 44 (6): 531-542. Song S.Z., et al.
  2. Targeting the myostatin signaling pathway to treat muscle loss and metabolic dysfunction. J Clin Invest. (2021) 131 (9): e148372. Lee SJ.

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