Human nephrines urine ELISA kit is intended for detecting in vitro quantitative concentrations of nephrines (metanephrine or normetanephrine) in human urine samples. This assay has a minimum detection limit of 13.0 pg/ml (MN) and 23.0 pg/ml (NMN).
Metanephrine and normetanephrine both contain a common structure of a methylated catechol ring (3-methoxy 4-hydroxy phenyl) in conjunction with an ethylamine group. Both of these are also O-methylated metabolites of the catecholamines epinephrine (E) and norepinephrine (NE) respectively. However, metanephrine is known to have a higher molecular weight of approx. 197 g/mol whereas NM is 183 g/mol. The only reason for this difference is because it contains a methylated amino group.
For clinical medicine metanephrine and normetanephrine have played essential roles in helping to identify individuals that could be possible suspects for possessing tumors of chromaffin (e.g. pheochromocytoma and paraganglioma). These metanephrines are also helpful as markers for extraneuronal metabolism of the parent catecholamines.
Both of the metabolites are routinely analysed in either plasma or urine samples, where it is only recently there has been an emergence of accurate and reliable procedure for determining plasma free metanephrines. Whereas the measurement of urine conjugated metanephrines have been used for quite a long time now and also is a very well established procedure.
NEPHRINES URINE ELISA KIT CONTENT
All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.
- Reaction Tubes.
- Wash Buffer Concentrate (50x).
- Enzyme Conjugate.
- TMB Substrate.
- Stop Solution.
- Metanephrine (MN) Microtiter Strips.
- Normetanephrine (NMN) Microtiter Strips.
- Metanephrine Antiserum.
- Normetanephrine Antiserum.
- Standards (A-F): Concentrations 0, 20, 60, 200, 600, 2,000 ng/ml (MN) and 0, 30, 90, 300, 900, 3,000 ng/ml (NMN).
- Control 1 + Control 2.
- Acylation Concentrate.
- Acylation Diluent.
- Acylation Buffer.
- Hydrochloric Acid.
The minimum sensitivity level of metanephrine/normetanephrine (nephrines) using current nephrines urine ELISA kit was approximately 13.0 pg/ml (M) or 23.0 pg/ml (MN). The dynamic range for this assay is 20.0 – 2,000 pg/ml (MN) and 30.0 – 3,000 pg/ml (NMN).
– Nephrines Urine (Normetanephrine, Metanephrine): ELISA
– Analytical Sensitivity: MN – LOD (8.6 ng/ml), LOB (6.0 ng/ml) and LOQ (10.5 ng/ml), NMN – LOD (14.7 ng/ml), LOB (10.4 ng/ml) and LOQ (16.2 ng/ml).
– Analytical Specificity (Cross Reactivity): Derivatized metanephrine (100%), Derivatized normetanephrine (100%).
– Intra Assay Precision: 9 – 12% (MN) and 9 – 13% (NMN)
– Inter Assay Precision: 16 – 17% (MN) and 11 – 20% (NMN)
– Linearity: 46.2 – 204 ng/ml (Metanephrine) and 98.8 – 347 ng/ml (Normetanephrine)
– Recovery: 85 – 113% (Metanephrine) and 93 – 111% (Normetanephrine)
- Deconjugated urinary metanephrine, normetanephrine and 3-methoxytyramine in laboratory diagnosis of pheochromocytoma and paraganglioma. Physiol Res. (2015) 64 Suppl 2: S313-22. Bílek R., et al.
- Diagnostic accuracy of free and total metanephrines in plasma and fractionated metanephrines in urine of patients with pheochromocytoma. Eur J Endocrinol. (2010) 162 (5): 951-60. Grouzmann E., et al.
- Urinary and plasma catecholamines and urinary catecholamine metabolites in pheochromocytoma: diagnostic value in 19 cases. Clin Chem. (1994) 40 (2): 250-6. Gerlo E.A. and Sevens C.
- Precisely wrong? Urinary fractionated metanephrines and peer-based laboratory proficiency testing. Clin Chem. (2005) 51 (2): 472-3. Singh R.J., et al.
- Full Name: Nephrines Urine (Metanephrine/Normetanephrine) ELISA Kit
- Reactivity: Human
- Sample Type: Urine
- Sensitivity: 13.0 pg/ml (M) and 23 pg/ml (NM)