Mycoplasma Pneumoniae IgG ELISA Kit

INTENDED USE

Human Mycoplasma pneumoniae IgG ELISA kit is a procedure for measuring in-vitro levels of human IgG antibodies against Mycoplasma pneumoniae (Mycoplasma-IgG) in plasma and serum. This assay has a minimum analytical sensitivity limit of 1.12 U/ml.

BACKGROUND

Mycoplasma pneumoniae are a class of mollicutes which are responsible for causing the human disease mycoplasma pneumonia. There are a form of atypical bacterial pneumonia which are known to be related to the cold agglutinin disease. They are parasitic species that are void of periplasmic space and a cell wall, consist of a reduce genome, have limited metabolic activity and are the smallest self-replicating organisms.

Mycoplasma pneumoniae cells are made up of an elongated shape. It can also cause tracheobronchitis, pharyngitis, otitis or other or community-acquired pneumonia, however, it may remain totally asymptomatic.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Mycoplasma pneumoniae antigen coated microtiter strips.
• Positive Control (Calibrator D).
• Negative Control (Calibrator A).
• Cut-Off Standard (Calibrator B).
• Weak Positive Control (Calibrator C).
• Enzyme Conjugate.
• Sample Diluent.
• TMB Substrate.
• Washing Buffer (10x).
• Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of human IgG antibodies to Mycoplasma pneumoniae using current Mycoplasma IgG ELISA kit was 1.12 U/ml. The dynamic range for this assay is 1.0 – 100.0 U/ml.

REFERENCES

1. IgGresponse to Mycoplasma pneumoniaein patients with community-acquired pneumonia determined by ELISA. APMIS. (1988) 96 (7): 605-10. Hirschberg L., et al.
2. The atypical pneumonias: clinical diagnosis and importance. Clin Microbiol Infect. (2006) 12 Suppl 3: 12-24. Review. Cunha B.A.
3. Evaluation of four commercial IgG- and IgM-specific enzyme immunoassays for detecting Mycoplasma pneumoniae-antibody: comparison with particle agglutination assay. J Korean Med Sci. (2007) 22 (5): 795-801. Yoo S.J., et al.
4. IgG-mediated haemolysis masquerading as cold agglutinin-induced anaemia complicating severe infection with mycoplasma pneumoniae. Scand J Haematol. (1985) 35 (3): 264-9. Louie E.K., et al.
5. Preparative electroelution of specific protein antigens from Mycoplasma pneumoniae: use in an enzyme-linked immunosorbent assay (ELISA). Ann Inst Pasteur Microbiol. (1988) 139 (5): 589-603. Papierok G., et al.

ADDITIONAL INFORMATION

• Full Name: Mycoplasma Pneumoniae IgG ELISA Kit
• Reactivity: Human
• Sample Type: Serum, Plasma
• Sensitivity: 1.12 U/ml

OTHER RELATED ELISA KITS

• Mycoplasma Pneumoniae-IgM ELISA Kit
• Candida Albicans-IgG ELISA Kit
• Aspergillus Fumigatus-IgG ELISA Kit