Mumps IgM ELISA Kit

Full Name: Mumps IgM ELISA Kit
Reactivity: Human
Sample Type: Plasma, Serum
Sensitivity: 1.23 U/ml


Mumps is a contagious infection that is caused by a virus type which is known as paramyxovirus. It can be passed from one individual to another through nasal secretion, saliva and even close personal contacts. The primary signs is the presence of swollen salivary glands which often causes the cheeks to puff out. There are three sets of salivary glands on each side of the face, found to be located below and behind the ears.

Mumps has now become less frequent since the introduction of the measles, mumps and rubella (MMR) vaccine within the UK and many other developed countries. It can however, be found in children that have not yet received the vaccine. Recently, procedures such immunofluorescence, enzyme immunoassay and radio immunoassay have become more popular in detecting mumps in the laboratory.


Human mumps IgM ELISA kit is intended for analysing in-vitro concentrations of human IgM antibodies to mumps virus (mumps-IgM) using either plasma or serum. This assay has a minimum analytical sensitivity limit of 1.23 U/ml.


All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

  • Mumps antigen coated microtiter strips.
  • Enzyme Conjugate.
  • TMB Substrate.
  • Sample Diluent.
  • Washing Buffer (10x).
  • Stop Solution.
  • Negative Control (Calibrator A).
  • Positive Control (Calibrator D).
  • Cut-Off Standard (Calibrator B).
  • Weak Positive Control (Calibrator C).


The minimum detection sensitivity level of human IgM antibodies against mumps virus (mumps-IgM) using this mumps IgM ELISA kit was 1.23 U/ml. The dynamic range for this assay is 1.0 – 125.0 U/ml.


– Intra-Assay-Precision: 8.7 %
– Inter-Assay-Precision: 6.8 %
– Inter-Lot-Precision: 4.2 – 9.5 %
– Analytical Sensitivity: 1.23 U/mL
– Recovery: 121 – 128 %
– Linearity: 78 – 135 %
– Cross-Reactivity: No cross-reactivity to Measles and Varicella Zoster.
– Interferences: No interferences to bilirubin up to 0.3 mg/mL, riglycerides up to 5.0 mg/mL and hemoglobin up to 8.0 mg/mL.
– Clinical Specificity: 75 %
– Clinical Sensitivity: 100 %


  1. Comparison of automated chemiluminescence immunoassays with capture enzyme immunoassays for the detection of measles and mumps  IgM antibodies in serum. J Virol Methods. (2014) 196: 15-7. Haywood B., et al.
  2. Enzyme-linked immunosorbent assay for mumps IgM antibody: comparison of IgM capture and indirect IgM assay. J Virol Methods. (1985) 12 (3-4): 303-11. Sakata H., et al.
  3. Comparison of chemiluminescent immunoassay and ELISA for measles IgG and IgM. APMIS. (2015) 123 (8): 648-51. de Ory F., et al.
  4. Incidence and persistence of mumps-specific IgM and IgA in the sera of mumps patients. Scand J Infect Dis. (1974) 6 (2): 125-9. Bjorvatn B.
  5. Comparison of mumps IgM ELISAs in acute infection. J Clin Virol. (2007) 38 (2): 153-6. Krause C.H., et al.


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