Measles IgG ELISA Kit

Full Name: Measles IgG ELISA Kit
Reactivity: Human
Sample Type: Serum, Plasma
Sensitivity: 1.35 U/ml

BACKGROUND

Measles (also called rubeola), is an extremely contagious viral disease which can be transmitted by respiratory secretions. The measles virus is a negative sense, single stranded enveloped RNA virus that belongs to the Morbillivrus (Paramyxovirdae family).

Measles virus can infect cells in the lungs and those that are at the back of the throat, causing symptoms such as sore throat, dry cough, a runny nose, tiny white spots inside the mouth, a high fever, red eyes and a characteristic rash. Two of the most common methods which are used for detecting this infection are: antibody testing (IgG and IgM) and viral isolation detection (RT-PCR).

INTENDED USE

Human measles IgG ELISA kit is intended for analysing in-vitro levels of human IgG antibodies against measles virus (measles-IgG) using plasma or serum. This assay has a minimum analytical sensitivity limit of 1.35 U/ml.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

Measles antigen coated microtiter strips.
Cut-Off Standard (Calibrator B).
Positive Control (Calibrator D).
Weak Positive Control (Calibrator C).
Negative Control (Calibrator A).
Enzyme Conjugate.
Sample Diluent.
TMB Substrate.
Washing Buffer (10x).
Stop Solution.

SENSITIVITY

The minimum detection sensitivity level of human IgG antibodies to measles virus (measles-IgG) using current measles virus IgG ELISA kit was 1.35 U/ml. The dynamic range for this assay is 1.0 – 250.0 U/ml.

ASSAY CHARACTERISTICS

– Intra-Assay-Precision: 12.5 %
– Inter-Assay-Precision: 6.9 – 15.8 %
– Inter-Lot-Precision: 2.7 – 12.2 %
– Analytical Sensitivity: 1.35 U/mL
– Recovery: 85 – 94 %
– Linearity: 75 – 129 %
– Cross-Reactivity: No cross-reactivity to Mumps and Varicella.
– Interferences: No interferences to triglycerides up to 5.0 mg/mL, hemoglobin up to 8.0 mg/mL and bilirubin up to 0.3 mg/mL.
– Clinical Specificity: 100 %
– Clinical Sensitivity: 97 %

REFERENCES

Comparison of chemiluminescent immunoassay and ELISA for measles IgG and IgM. APMIS. (2015) 123 (8): 648-51. de Ory F., et al.
High Concentrations of Measles Neutralizing Antibodies and High-Avidity Measles IgG Accurately Identify Measles Reinfection Cases. Clin Vaccine Immunol. (2016) 23 (8): 707-16. Sowers S.B., et al.
Detection of measles specific IgG in oral fluid using an FITC/anti-FITC IgG capture enzyme linked immunosorbent assay (GACELISA). J Virol Methods. (1999) 83 (1-2): 135-44. Nigatu W., et al.
Measles virus IgG avidity assay for use in classification of measles vaccine failure in measlese limination settings. Clin Vaccine Immunol. (2012) 19 (11): 1810-7. Mercader S., et al.
Electrochemical impedimetric immunosensor for the detection of measles-specific IgG antibodies after measles infections. Biosens Bioelectron. (2013) 49: 32-8. Mashazi P., et al.

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