Lipocalin-2 ELISA Kit (NGAL)

Full Name: Lipocalin-2 ELISA Kit (NGAL)
Reactivity: Human
Sample Type: Cell Supernatant, Serum, Urine, PlasmaSensitivity:0.39 ng/ml

INTRODUCTION

Licocalin-2 (LCN2, NGAL) has been implicated in important roles that are linked to the innate immunity and apoptosis. It interacts with MUC1, TLR4 and CCL14, which are involved in the innate immune system. It also interacts with OPN1LW, which is thought to be involved in apoptosis. It has been shown that cell-surface NGAL (LCN2) is a biomarker of various cancers and tumor development; this proposed role reflects the similarities between NGAL expression patterns and those of tumor cells described by special staining methods in clinical pathology laboratories.

When the innate immune system encounters an assault from micro-organisms this leads to TLRs being incited, this results in licocalin-2 being expressed and secreted. The function is to reduce and limit the growth of micro-organisms through a process of sequestrating the iron containing siderophones. The expression can be stimulated by IL-1, LPS and TNF-alpha. In response to the expression of licocalin-2, the Fe3+ ions are sequestered in the cells with the help of a metal transporter. The amount of Fe3+ ions that are sequestered depends on how much iron there is and what type it is. For example, ferrous iron is specifically found as part of heme proteins, whereas ferric iron has a less defined structure and instead binds to a variety of proteins including transferrin receptors and metallothioneins.

There is evidence to indicate that licocalin-2 can exist as both either as a paracrine and autocrine adipokine, displaying anti-inflammatory properties. Also, it can be described as a neutrophil gelatinase-associated lipocalin (NGAL) and it was initially discovered as secondary granules present in human neutrophils. Other names that it is called include oncogene 24p3, uterocalin, neu-related lipocalin and siderocalin. Despite being released from the same cell, it has been shown to have different biological activities.

INTENDED USE

Human lipocalin-2 ELISA kit is designed for measuring concentrations of lipocalin-2 (NGAL, LCN2) in human serum, urine, plasma and cell supernatant samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• Microtitre Strips (96 Wells): Coated with mouse monoclonal antibody against licocalin-2 .
• 10x Wash Buffer.
• 5x Assay Buffer.
• 100x Detection Antibody Buffer: Labelled biotin polyclonal antibody.
• Standard: Recombinant lyophilized.
• 200x STP-HRP Solution.
• Substrate Solution.
• Stop Solution.

TYPICAL RESULTS

For the following lipocalin-2 ELISA kit it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.39, 1.56, 3.12, 6.25, 12.5, 25 ng/ml.

ASSAY CHARACTERISTICS

– Sensitivity: 0.39 ng/ml.
– Assay Range: 0.39 – 25 ng/ml.
– Specificity: No interference or cross reaction observed.
– Other Names: Lipocalin-2, NGAL, LCN2, Siderocalin, 24p3, Neutrophil lipocalin (NL), MSFI.

REFERENCES

1. Serum lipocalin-2, cathepsin S and chemerin levels and nonalcoholic fatty liver disease. Mol Biol Rep. (2014) 41 (3): 1317-23. Ye Z, et al.
2. More than a simple biomarker: the role of NGAL in cardiovascular and renal diseases. Clin Sci (Lond). (2018) 132 (9): 909-923. Buonafine M., et al.
3. Neutrophil gelatinase-associated lipocalin (NGAL) for the prediction of acute kidney injury in chronic kidney disease patients treated with primary percutaneous coronary intervention. Int J Cardiol Heart Vasc. (2020) 32: 100695. Banai A, et al.
4. NGAL protects against endotoxin-induced renal tubular cell damage by suppressing apoptosis. BMC Nephrol. (2018) 19 (1): 168. Han M, et al.

OTHER RELATED ELISA KITS

• Neutrophil ELISA Kit
• Galactin-3 ELISA Kit
• Haptoglobin ELISA Kit