LDHA ELISA Kit (L-Lactate Dehydrogenase A Chain)

INTENDED USE

Human LDHA ELISA kit can measure concentrations of L-lactate dehydrogenase A chain (LDHA, LDHM, cell proliferation-inducing gene 19 protein, LDH-A) present is plasma, serum, biological fluids or tissue homogenate samples.

INTRODUCTION

LDHA encodes the L-lactate dehydrogenase A chain, a homotetrameric enzyme that catalyzes the anaerobic conversion of pyruvate to L-lactate with concomitant nicotinamide adenine dinucleotide (NAD+) regeneration. This terminal glycolytic reaction constitutes the major pathway for metabolic flux during ischemia, sepsis, or other states of tissue oxygen debt. Moreover, aggressive tumor cells preferentially utilize cytoplasmic LDHA despite oxygen availability (“Warburg effect”), reflecting a proliferative switch toward lactate production over oxidative phosphorylation.

Ubiquitously expressed across tissues, LDHA can translocate to the nucleus and modulate transcription—potentially regulating glycolytic genes during early differentiation or in response to cellular stressors. Furthermore, via HIF1α activation, hypoxic conditions induce LDHA transcription to levels disproportionate with other glycolytic enzymes, indicating rate-limiting control over anaerobic metabolism. LDHA expression correlates with tumor progression, metastasis, and poor prognosis across diverse malignancies.

Selective inhibitors and monoclonal antibodies targeting LDHA elicit promising antiproliferative, proapoptotic, and antimetastatic responses in preclinical cancer models, likely by intervening in phosphoglycerate kinase-1 secretion or glutamine metabolism. Additionally, starving energy production via combined mitochondrial and LDHA blockade elicits profound oxidative stress. While pharmacological inhibition demonstrated safety in phase I trials, on-target muscle side effects remain a concern.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-LDHA antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this LDHA ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.781, 1.562, 3.125, 6.25, 12.5, 25, 50 ng/ml.
Reactivity: Human
Sensitivity: 0.469 ng/ml
Range: 0.781 – 50 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for LDHA, no cross reactivity or interference between LDHA and analogues was detected.
– Recovery: Serum (85 – 100%), EDTA Plasma (89 – 102%), Heparin Plasma (85 – 98%).
– Linearity: Serum (93 – 102%), EDTA Plasma (85 – 98%), Heparin Plasma (80 – 97%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. Protein arginine methyltransferase 3 promotes glycolysis and hepatocellular carcinoma growth by enhancing arginine methylation of lactate dehydrogenase A. Clin Transl Med. (2022) 12 (1): e686. Lei Y., et al.
2. LDHA promotes osteoblast differentiation through histone lactylation. Biophys Res Commun. (2022) 615: 31-35. Nian F., et al.
3. Yeast cell-based analysis of human lactate dehydrogenase isoforms. J Biochem. (2015) 158 (6): 467-76. Mohamed L.A., et al.
4. LDHA-Associated Lactic Acid Production Blunts Tumor Immunosurveillance by T and NK Cells. Cell Metab. (2016) 24 (5): 657-671. Brand A., et al.

ADDITIONAL INFORMATION

• Full Name: LDHA ELISA Kit (L-Lactate Dehydrogenase A Chain)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 0.469 ng/ml

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