IL20RA ELISA Kit (Interleukin-20 Receptor Subunit Alpha)

INTENDED USE

Human IL20RA ELISA kit can measure concentrations of interleukin-20 receptor subunit alpha (IL20RA, cytokine receptor class-II member 8, CRF2-8, class II cytokine receptor ZCYTOR7) present is plasma, serum, biological fluids or tissue homogenate samples.

INTRODUCTION

IL20RA (Interleukin 20 receptor subunit alpha) encodes one member of the heterodimeric receptor complex that mediates signaling of the IL-10 cytokine family members IL-19, IL-20, and IL-24 to regulate inflammatory responses. While exhibiting some overlapping activities, these cytokines drive inflammatory or inhibitory effects depending on receptor engagement and cell types involved. As one receptor component conferring ligand specificity, IL20RA expression patterns and signaling modulation presents opportunities for tailored immune intervention.

The type I transmembrane glycoprotein IL20RA combines with IL20RB to permit IL-19, IL-20, and IL-24 signaling. However, IL20RA alone binds IL-19 and IL-24. Following engagement, subsequent phosphorylation cascades involving JAK-STAT pathways activate downstream transcription of genes governing proliferation, chemotaxis, apoptosis and immunity. For example, IL20RA-dependent IL-19 signaling stimulates monocytes but generally suppresses other leukocytes like T-cells when protected from counter-regulation. Consequently, the IL-20 subfamily cytokines display highly contextual outcomes.

IL-20 subfamily overexpression from keratinocytes may drive characteristic hyperplasia and pathogenesis. Genetic variants in IL20RA also associate with plaque psoriasis susceptibility. Quantifying serum IL20RA levels by enzyme-linked immunosorbent assays may enable gauging disease severity while pharmacologically modulating availability or activity could provide routes for therapeutic intervention against recalcitrant autoimmune disorders.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti-IL20RA antibody.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this IL20RA ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 0.313, 0.625, 1.25, 2.5, 5, 10, 20 ng/ml.
Reactivity: Human
Sensitivity: 0.188 ng/ml
Range: 0.313 – 20 ng/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IL20RA, no cross reactivity or interference between IL20RA and analogues was detected.
– Recovery: Serum (90 – 99%), EDTA Plasma (89 – 103%), Heparin Plasma (94 – 104%).
– Linearity: Serum (94 – 104%), EDTA Plasma (83 – 97%), Heparin Plasma (80 – 90%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. IL20RA signaling enhances stemness and promotes the formation of an immunosuppressive microenvironment in breast cancer. Theranostics. (2021) 11 (6): 2564-2580. Gao W., et al.
2. Interleukin 20 receptor A expression in colorectal cancer and its clinical significance. Peer J. (2021) 9: e12467. Liu R., et al.
3. The Interleukin-20 Cytokine Family in Liver Disease. Front Immunol. (2018) 9: 1155. Caparrós E. and Francés R.

ADDITIONAL INFORMATION

• Full Name: IL20RA ELISA Kit (Interleukin-20 Receptor Subunit Alpha)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 0.188 ng/ml

OTHER RELATED ELISA KITS

• ICTP ELISA Kit
• OGG1 ELISA Kit
• MSTN ELISA Kit