IL-17F ELISA Kit (Interleukin 17F)

INTENDED USE

Human Interleukin 17F ELISA kit can measure concentrations of interleukin 17F (IL-17F) present is plasma, serum, biological fluids or tissue homogenate samples.

INTRODUCTION

Interleukin 17F (IL-17F) is a proinflammatory cytokine playing key roles in immune defense against extracellular bacteria and fungi as part of the Th17 immune response. Secreted by activated T cells and innate lymphocytes, IL-17F signals tissue cells to upregulate antimicrobial proteins, recruit neutrophils, and trigger production of other inflammatory mediators.

The IL-17 family of cytokines, elicit protective effects against pathogen infections – especially at mucosal barrier surfaces like the lungs and gut. Binding its receptor prompts tissue cells to secrete cytokines, chemokines, and antimicrobial peptides while also increasing mucus production. This fuels inflammation and direct microbe elimination. IL-17F spurs neutrophil expansion and migration as well to deploy phagocytic infection-fighting cells.

However, unchecked IL-17F activity can enable harmful inflammatory pathology and autoimmunity instead of balanced immune protection. Overactive Th17 cell populations and uncontrolled IL-17F production associate with numerous inflammatory disease states like psoriasis, rheumatoid arthritis, asthma, inflammatory bowel disease, and multiple sclerosis. Therapeutics blocking IL-17F and Th17 signaling alleviate symptoms in many of these disorders.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti- IL-17F.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this Interleukin 17F ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml.
Reactivity: Human
Sensitivity: 9.375 pg/ml
Range: 15.625 – 1000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for IL-17F, no cross reactivity or interference between IL-17F  and analogues was detected.
– Recovery: Serum (87 – 101%), EDTA Plasma (95 – 105%), Heparin Plasma (90 – 104%).
– Linearity: Serum (85 – 103%), EDTA Plasma (84 – 98%), Heparin Plasma (81 – 97%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. IL-17F: regulation, signaling and function in inflammation. Cytokine. (2009) 46 (1): 7-11. Chang S.H. and Dong C.
2. Functional specialization of interleukin-17 family members. Immunity. (2011) 34 (2): 149-62. Iwakura Y., et al.
3. Potential involvement of IL-17F in asthma. J Immunol Res. (2014) 2014: 602846. Ota K., et al.
4. Variable roles of interleukin-17F in different cancers. BMC Cancer. (2022) 22 (1): 54. Mikkola T., et al.

ADDITIONAL INFORMATION

• Full Name: IL-17F ELISA Kit (Interleukin 17F)
• Reactivity: Human
• Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
• Sensitivity: 9.375 pg/ml

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