Folate Receptor Alpha ELISA Kit (FOLR1)

Full Name: Folate Receptor Alpha ELISA Kit (FOLR1)
Reactivity: Human
Sample Type: Plasma, Tissue Homogenates, Serum, Biological Fluids
Sensitivity: 9.375 ng/ml

INTRODUCTION

FOLR1, known as folate receptor alpha, is a cell surface glycoprotein that internalizes dietary folic acid for critical intracellular metabolic roles. Highly expressed in epithelial cells lining folate-absorbing lumens, including prominently in certain cancer types, FOLR1 offers a portal for targeting conjugated anti-tumor pharmaceuticals.

Certain membrane-bound proteins facilitate uptake of small molecule nutrients like folic acid, which requires an extracellular-to-intracellular transport mechanism given its hydrophilic nature. By endocytosing bound vitamins, FOLR1 in epithelial membranes retrieves needed nutrients from external compartments. Once inside cells, folic acid serves as a formyl group donor to enable one-carbon transfers essential for DNA and RNA synthesis along with amino acid production, thus facilitating multiple critical metabolic pathways especially in rapidly dividing cells.

This dependency likely explains FOLR1 upregulation in over 70% of ovarian cancer and roughly 30-40% of lung and breast cancers to fuel relentless proliferation demands. By conjugating anti-tumor drugs to folic acid, medics can exploit folate receptor overexpression on cancer cells for targeted therapy delivery while minimizing off-target toxicity. Multiple cytotoxic FOLR1-directed agents now undergo clinical evaluation, with promising treatment responses yet also unique toxicity concerns.

INTENDED USE

Human Folate Receptor Alpha ELISA kit can measure concentrations of FOLR1 (Folate Receptor Alpha, FR-Alpha, folbp, adult folate-binding protein, FBP) present is plasma, serum, biological fluids or tissue homogenate samples.

CONTENT

All reagents supplied need to be stored at 2 °C – 8 °C, unopened reagents will retain reactivity until expiration date. Do not use reagents beyond this date.

• One 96-Well Plate: Pre-coated with anti- FOLR1.
• Standards: Lyophilized recombinant.
• Sample/Standard Dilution Buffer.
• Biotinylated-labelled Antibody.
• Antibody Dilution Buffer.
• HRP-Streptavidin Conjugate (SABC).
• SABC Dilution Buffer.
• TMB Substrate.
• Wash Buffer (25x).
• Plate Sealer.
• Product Instructions.

TYPICAL RESULTS

For this Folate Receptor Alpha ELISA kit, it is recommended that a standard curve is generated for each assay carried out.

Standard Curve: 0, 15.625, 31.25, 62.5, 125, 250, 500, 1000 pg/ml.
Reactivity: Human
Sensitivity: 9.375 pg/ml
Range: 15.625 – 1000 pg/ml
Principle: Sandwich, Double Antibody
Application: Research Use Only.

ASSAY CHARACTERISTICS

– Specificity: Highly specific for FOLR1, no cross reactivity or interference between FOLR1 and analogues was detected.
– Recovery: Serum (88 – 101%), EDTA Plasma (92 – 104%), Heparin Plasma (85 – 101%).
– Linearity: Serum (85 – 105%), EDTA Plasma (83 – 99%), Heparin Plasma (83 – 98%).
– Precison Intra-Assay: CV < 8%
– Precison Inter-Assay: CV < 10%

REFERENCES

1. Exploiting the folate receptor α in oncology. Nat Rev Clin Oncol. (2020) 17 (6): 349-359. Scaranti M., et al.
2. Emerging roles for folate receptor FOLR1 in signaling and cancer. Trends Endocrinol Metab. (2022) 33 (3): 159-174. Nawaz F.Z. and Kipreos E.T.
3. Folate receptor alpha in ovarian cancer tissue and patient serum is associated with disease burden and treatment outcomes. Br J Cancer. (2023) 128 (2): 342-353. Bax H.J., et al.
4. Protein-Labeling Fluorescent Probe for Folate Receptor α. Anal Chem. (2023) 95 (30): 11535-11541. Fang P.W., et al.

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